NanoBRET® 590 Dyes
Develop Novel NanoBRET® Tracers to Enable Target Engagement Assays for New Targets
- Leverage the same NanoBRET®-branded energy acceptors used to create most Promega NanoBRET® tracers
- Couple NanoBRET® 590 SE Dyes to base compounds containing a reactive amine group
- Create three unique tracer structures from a common starting material using the three linker variants of the NanoBRET® 590 SE Dye
- Synthesize tracers with an amine orthogonal coupling chemistry using Azide-Alkyne Click chemistry and the NanoBRET® 590 Azide Dye
Catalog Number:
Choose a dye
Size
Catalog Number: N5000
Catalog Number: N5001
Catalog Number: N5100
Catalog Number: N5200
Catalog Number: N5201
Catalog Number: N5300
Catalog Number: N5301
標的タンパク質に対するNanoBRET™ TEトレーサーの作成
早期創薬ワークフローに NanoBRET™ 590 Dye を利用
DNAコードライブラリー（DEL）は、比類のない化学的多様性を提供し、新規薬剤モダリティの出発点となり得ます。Cell Chemical Biology 誌に掲載された論文では、DELリガンドの二官能性を利用し、ライブセル標的結合研究用のBRETプローブを生成するプラットフォームとしてのワークフローが紹介されています。DEL と BRET技術の融合は、ライブセル環境におけるヒット・to・リード研究を加速します。
詳細については : Teske, K.A. et al. (2023) DELs enable the development of BRET probes for target engagement studies in cells. Cell Chem. Biol. 30, 1–12.
NanoBRET™ Target Engagement (TE) Assay は、インタクトな細胞内における標的タンパク質への化合物の結合性を測定します。このターゲット・エンゲージメント・アッセイは、NanoBRET™ システムに基づいており、生きた細胞内で分子の近接性を測定するために設計されたエネルギー移動技術です。NanoBRET™ Target Engagement Assayは、細胞内でNanoLuc® 融合タンパク質に可逆的に結合したNanoBRET™トレーサーを競合的に置換することにより、被験物質の見かけの親和性を分析します。
プロメガでは、様々なタンパク質クラスの多数のタンパク質ターゲットに対して、既に多くのTEアッセイを開発しています。ご希望のターゲットに対する既存のNanoBRET™ TE アッセイが無い場合は、NanoBRET™ 590 Dyes を用いて新規トレーサーを開発し、新しいターゲットに対するNanoBRET™ TE アッセイ構築にご利用ください。
NanoBRET™ TEアッセイのしくみ
NanoBRET™ TEアッセイの概要. パネル A. 細胞透過性の蛍光 NanoBRET™ トレーサーを用いて、化合物の結合を競合形式で測定。試験化合物の結合により、インタクトな細胞内で標的タンパク質とトレーサー間のNanoBRET™シグナルが消失。パネル B. NanoBRET™ トレーサーの各標的タンパク質に対する親和性を測定。試験化合物による標的タンパク質の関与を解析するために、NanoBRET™ トレーサーの用量反応曲線のEC50値に近い一定濃度のNanoBRET™ トレーサーで細胞を処理。 パネル C. 被験物質との親和性を測定するために、一定濃度（EC50-EC80）のトレーサー存在下、様々な濃度の被験物質で細胞を滴定。
トレーサー開発を加速するリンカー付き色素
NanoBRET™ 590 Dyes は新規細胞透過性トレーサーの開発に使用でき、プロメガから販売されている各種 NanoBRET™ トレーサーの開発に使用されているものと同じです。NanoBRET™ 590 Dyes は一貫して強い細胞透過性を示し、エネルギー移動のために NanoLuc® Luciferase と最適なスペクトルのオーバーラップを示します。NanoBRET™ 590 SE Dyes の3種類のリンカーバリアント（パネル A-C）があり、共通の中間体からトレーサー候補のパネルを調製することができます。また、NanoBRET™ 590 Azide Dye（パネルD）は、アジド-アルキン クリックケミストリーによるトレーサー合成を可能にし、アミン直交カップリングケミストリーを提供します。
NanoBRET™ Dyes の構造 (赤色は蛍光色素)。パネル A. NanoBRET™ 590 SE Dye (MW: 426.2g/mol). パネル B. NanoBRET™ 590 C4 SE Dye (MW: 511.3g/mol). パネル C. NanoBRET™ 590 PEG(O4) SE Dye (MW: 673.5g/mol). パネル D. NanoBRET™ 590 C3 Azide Dye (MW: 411.2g/mol).
トレーサー作成からアッセイへ
目的の標的タンパク質に対する既存の NanoBRET™ TE アッセイがない場合、NanoBRET™ 590 Dyes を用いて新規トレーサーを開発し、新しいターゲットに対する NanoBRET™ TE アッセイを可能にすることができます。
NanoBRET™ TE アッセイ開発ワークフロー
NanoBRET™ TE アッセイ開発のワークフロー. NanoBRET™ TE アッセイを開発する3つのステップは以下の通りです： (1) 標的タンパク質と結合する蛍光トレーサーの開発、(2) 標的タンパク質と NanoLuc® Luciferase の融合体を細胞内で発現するベクターの構築、(3) NanoBRET™ TE アッセイ試薬を用いた NanoBRET™ TE 細胞内アッセイ の最適化。完全なワークフローを完成させるには、さらに試薬が必要です。詳しくは、よく使用される製品（下記）をご覧ください。
文献紹介
WD-Repeat-Containing Protein 5 (WDR5) でデグレイダー（分解剤）の設計、合成、および評価
本研究では、2つのWIN部位結合足場に基づく2つの多様なWDR5分解剤シリーズの設計、合成および評価を行い、リンカーの性質と長さが分解効果に強く影響することを示しています。
tau チューブリンキナーゼ（TTBK1およびTTBK2）のモジュレーションが繊毛形成に影響する
これらのキナーゼの生物学的重要性を考慮して、著者らは標的ライブラリーを設計し、そこから細胞内でTTBK1とTTBK2に関与し、その下流のシグナル伝達を阻害するいくつかの化学ツールを同定しています。
エネルギー移動を用いた細胞内標的関与の定量的リアルタイム測定
バイオルミネッセンス共鳴エネルギー移動（BRET）は、定量的な測定基準や速度論的解析が必要な場合に、細胞内標的関与のためのソリューションを提供します。
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
NanoBRET® 590 SE |
N500A | 1 × 5mg |
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
NanoBRET® 590 SE |
N500B | 1 × 25mg |
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
NanoBRET® 590 Azide |
N510A | 1 × 5mg |
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
NanoBRET® 590 PEG(O4)-SE |
N520A | 1 × 5mg |
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
NanoBRET® 590 SE |
N500A | 1 × 5mg |
NanoBRET® 590 PEG(O4)-SE |
N520A | 1 × 5mg |
NanoBRET® 590 C4 SE |
N530A | 1 × 5mg |
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
NanoBRET® 590 C4 SE |
N530A | 1 × 5mg |
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
NanoBRET® 590 C4 SE |
N530B | 1 × 25mg |
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
技術記事
- Finding the Nexus: A New Technique for Studying Kinase Target Engagement in Live Cells
- Advancing Biomedical Research with Quality Chemical Probes
- Blog Article: The Surprising Landscape of CDK Inhibitor Selectivity in Live Cells
- Development of a HiBiT Peptide-Based NanoBRET Ligand Binding Assay for Galanin Receptor 1 in Live Cells
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