GoTaq® MDx Hot Start Polymerase
Inhibits Polymerase Activity at Temperatures below 70°C
- High-concentration and glycerol-free formulations also available
- All formulations supplied at ≥5u/μl, except for high-concentration formulation, which is supplied at ≥50u/μl
- Custom formats and formulations
- Manufactured under cGMP for consistent, lot-to-lot performance
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated.
Catalog Number:
Choose an option
Size
Catalog Number: D6001
Catalog Number: D6005
Catalog Number: D6006
Catalog Number: D6008
Catalog Number: D6101
Catalog Number: D6201
Consistent and Robust Amplification
GoTaq® MDx Hot Start Polymerases にはポリメラーゼ活性をブロックする特殊な抗体が結合したGoTaq® MDx DNA Polymerase が含まれています。ポリメラーゼ活性は、増幅反応の最初の94–95°C、2分間の変性ステップで回復します。70°C以下でポリメラーゼ活性を阻害するホットスタートPCRにより、簡便な室温での反応セットアップが可能になります。また、ホットスタートPCRによりプライマーダイマーや副産物を低減し、収量が増加する場合もあります。グリセロールを除去した製品は、原料としてさらに手を加えて製品化する場合や、凍結乾燥する際に有用です。
- ロット間のばらつきの無い安定した性能を保証するため、品質管理システムの下、妥当性が確認された器具、工程、方法によりcGMP製造されています。
- DNA混入の低いことを細菌、カビ、哺乳類DNAに対しQCテストしています。
- 融通の利く構成ですので、カスタム化にも対応いたします。
GoTaq® MDx Hot Start Polymeraseは一般的な実験に用いる広範な用途に向けた製品です。また、該当する国の法令が許可する遺伝子診断アッセイの構成品として使用することができます。別途ライセンスや使用料を要求することはありません。
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.
That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
Consistent Performance
Variance of 12 separately manufactured lots of product from manufacture target concentration
GoTaq® MDx DNA Polymerase products are manufactured under cGMP using validated equipment, processes and methods under a certified Quality System to ensure consistent product performance lot-to-lot.
Reduce nonspecific amplification with hot-start PCR using GoTaq® MDx Hot Start Polymerase
High Quality
| GoTaq® MDx DNA Polymerase products meet stringent specifications for: | |
|---|---|
| Purity | ≥90% (Reverse Phase- HPLC) |
| DNA Contamination | One unit contains less than:
|
| Nuclease Contamination | Undetectable exonuclease, endonuclease, nicking and ribonuclease activity |
| Performance | Quantitative amplification of DNA target. Amplification of hot-start model template produces a single 1.5kb band without extraneous bands (GoTaq® MDx Hot Start Polymerase formulation only) |
| Stability | Stable for 24 months from date of manufacture |
Customization
Rely on an Expert Partner
Choose a trusted partner with the robust and reliable reagents you need for your molecular assay and the experience to support you throughout your assay development process.
More than 30 years of manufacturing experience
Commitment to high-quality standards
- ISO 13485 & ISO 9001 certified
- cGMP-manufacturing capabilities
Consistent and reliable amplification products
Flexible, custom, made-to-order solutions available
To use GoTaq® MDx Hot Start Polymerase in a custom format or to distribute GoTaq® MDx Hot Start Polymerase, contact the Promega Custom Order Department to discuss specific requirements.
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 1 × 750μl | 25mM | View Product |
|
GoTaq® MDx Hot Start Polymerase |
D600A | 1 × 100u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 1 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 1 × 1ml | View Product |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 3 × 750μl | 25mM | View Product |
|
GoTaq® MDx Hot Start Polymerase |
D600B | 1 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 4 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 4 × 1ml | View Product |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 15 × 750μl | 25mM | View Product |
|
GoTaq® MDx Hot Start Polymerase |
D600B | 5 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 20 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 20 × 1ml | View Product |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 60 × 750μl | 25mM | View Product |
|
GoTaq® MDx Hot Start Polymerase |
D600B | 20 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 80 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 80 × 1ml | View Product |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 2 × 1.2ml | 25mM | View Product |
GoTaq® MDx Hot Start Polymerase |
D610A | 1 × 1,000u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890C | 2 × 5ml | View Product |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 3 × 750μl | 25mM | View Product |
GoTaq® MDx Hot Start Polymerase, Glycerol-Free |
D620A | 1 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 4 × 1ml | View Product |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
