PCR Optimization Kit
Preformulated PCR Master Mix Components to Determine Optimal PCR Amplification Conditions
- Use this kit to build your custom, Made-to-Order 2X PCR Master Mix
- Manufactured under cGMP for consistent, lot-to-lot PCR performance
- Preformulated buffers cover a wide variety of PCR performance capabilities
- Available with dUTP
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated.
Catalog Number:
Size
Catalog Number: D2381
Uncover Your Assay's Award-Winning Formula
PCR Optimization Kitには、エンドポイント、マルチプレックス、リアルタイム、GCリッチや阻害物質に強いPCRをカバーする予め調製された高品質バッファー(A–H)が含まれています。また、25mM MgCl2水溶液とGoTaq® MDx Hot Start Polymeraseが附属しており、目的とするPCRに適したバッファーの選択から、MgCl2と酵素濃度の最適化が迅速に行えます。cGMP製造されており、安定したPCR性能を提供します。
様々な条件検討に時間をかけることなく目的にあったPCR条件を設定できます。一度最適条件が決まれば、オーダーメードをご検討下さい。Made-to-Order 2X PCR Master Mixで注文を承っています。
Step 1
最適なPCRバッファーを選択します。高品質な、8種類の異なる組成のバッファーを用いてエンドポイント、マルチプレックス、リアルタイム、GCリッチあるいは阻害物質に強い増幅などを試します。目的のPCRで良い結果が得られたバッファー（数種類）を候補とします。
Step 2
ガードバンド試験を効率的に行う。Step 1で選択した候補バッファーを使い、求める性能規格を満たす組成を決定するためアニーリング温度、MgCl2濃度、酵素濃度を最適化します。
Step 3
ロバストな再現性のあるPCR増幅を行えます。最適化した組成で2X PCR Master Mixを弊社がcGMP製造いたします。
No-Hassle Customization
Once you identify your optimal PCR formulation, seamlessly transition into daily execution of your PCR assay by continuing your work with a Made-To-Order 2X PCR Master Mix or purchase the stand-alone buffer with MgCl2 and GoTaq® MDx Hot Start Polymerase. Simply tell us what your reaction formulation is (which buffer, how much MgCl2 and enzyme you used), and we’ll make your personalized 2X PCR Master Mix*.
*Minimum order quantity required for Made-To-Order 2X PCR Master Mixes.
These products are general purpose reagents intended for general laboratory use. They can be used as a component of molecular diagnostic assays where applicable country laws allow. Separate license or royalty payments are not required.
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.
That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
Protocols
Complete Protocol
Specifications
Catalog Number:
SDS
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使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
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