T4 DNA Ligase
Joins Two DNA Strands with Cohesive or Blunt Ends
- Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors)
- Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
- Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
Choose a concentration
Size
Catalog Number: M1801
Catalog Number: M1804
Catalog Number: M1794
- 平滑末端あるいは付着末端の2本鎖DNA分子を連結
さらに知りたい方は、プロトコール＆ガイド(英語).をご覧ください。
Units listed are Weiss units.
- Engler, M.J. and Richardson, C.C. (1982) In: The Enzymes, Boyer, P.D., ed., Academic Press, New York, NY.
Note: Units listed are Weiss units.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.
Source: Recombinant E. coli strain.
QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.
Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
T4 DNA Ligase 10X Buffer |
C126B | 1 × 200μl | View Product |
|
T4 DNA Ligase |
M180A | 1 × 100u |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
T4 DNA Ligase 10X Buffer |
C126A | 2 × 500μl | View Product |
|
T4 DNA Ligase |
M180B | 1 × 500u |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
T4 DNA Ligase 10X Buffer |
C126B | 1 × 200μl | View Product |
|
T4 DNA Ligase (HC) |
M179A | 1 × 500u |
SDS
Search for SDS分析証明書
使用制限
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.保存条件
Resources
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