T▼GATC A
A CTAG▲T
- 适用于基因组: 普洛麦格的适用于基因组的限制性内切酶通过实验证实能够保证在基因组分析实验中具有高效表现。
- Sensitive to dam methylation.
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.
T▼GATC A
A CTAG▲T
- 适用于基因组: 普洛麦格的适用于基因组的限制性内切酶通过实验证实能够保证在基因组分析实验中具有高效表现。
- Sensitive to dam methylation.
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer C 10X Buffer |
R003A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
BclI |
R665A | 1 × 1,000u |