DpnI
- Capable of digesting DNA in 15 minutes or less
Catalog Number:
Choose a concentration
Size
Catalog Number: R6231
Cut Site:
GmeA▼TC
CT▲meAG
Usage Note: DpnI requires N6-methylation of the adenine residue for activity, unlike its isoschizomers, MboI and Sau3AI.
Incubation Conditions: Buffer B. 37°C.
Source: Diplococcus pneumoniae.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-CORE™ |
|---|---|---|---|---|
| 50–75% | 100% | 75–100% | 50–75% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 116 | 87 | 0 | 15 | 7 | 22 |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer B 10X Buffer |
R002A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
DpnI |
R623A | 1 × 200u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
