EcoRI
- Capable of digesting DNA in 15 minutes or less
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Available at high concentration, containing 25,000 units of EcoRI at a concentration of 40–80u/μl
Catalog Number:
Choose a concentration
Size
Catalog Number: R6011
Catalog Number: R6017
Catalog Number: R4014
Cut Site:
G▼AATT C
C TTAA▲G
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Note: Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratios >25u/μg, in the absence of NaCl, in the presence of Mn2+, or under high pH conditions.
Incubation Conditions: Buffer H. 37°C.
Source: Escherichia coli RY 13.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 400mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | H | MULTI-CORE™ |
|---|---|---|---|---|---|
| 25–50% | 50–75% | 50–75% | 50–75% | 100% | 100%* |
*MULTI-CORE™ Buffer is not recommended due to potential star activity.
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 5 | 5 | 0 | 1 | 1 | 1 |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer H 10X Buffer |
R008A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
EcoRI |
R601A | 1 × 5,000u |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer H 10X Buffer |
R008A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
EcoRI |
R601J | 1 × 15,000u |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer H 10X Buffer |
R008A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396F | 1 × 1,250μl | 10mg/ml | View Product |
|
EcoRI (HC) |
R401A | 1 × 25,000u |
Resources
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