HinfI
- Restriction endonuclease from Haemophilus influenzae Rf
Catalog Number:
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Size
Cut Site:
G▼ANT C
C TNA▲G
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Note: For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
Incubation Conditions: Buffer B. 37°C.
Source: Haemophilus influenzae Rf.
Storage Buffer: 10mM Tris-HCl (pH 7.3), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-CORE™ |
|---|---|---|---|---|
| 50–75% | 100% | 75–100% | 75–100% | 50–75% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 148 | 72 | 21 | 6 | 27 | 10 |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer B 10X Buffer |
R002A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
HinfI |
R620A | 1 × 1,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer B 10X Buffer |
R002A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
HinfI |
R620B | 1 × 5,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
Resources
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