KpnI
- Capable of digesting DNA in 15 minutes or less
- Active and capable of digestion directly in GoTaq® Green Master Mix
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Catalog Number:
Choose a concentration
Size
Cut Site:
G GTAC▼C
C▲CATG G
Usage Notes:
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- KpnI recognizes the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, Acc65I.
- KpnI is not sensitive to overlapping dcm methylation, unlike its neoschizomer, Acc65I.
Incubation Conditions: Buffer J. 37°C.
Source: Recombinant E. coli strain.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 200mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | J | MULTI-CORE™ |
|---|---|---|---|---|---|
| 100%* | 25–50% | 25–50% | <10% | 100% | 75–100% |
*Buffer A is not recommended due to potential star activity.
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 2 | 8 | 0 | 1 | 1 | 0 |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer J 10X Buffer |
R009A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
KpnI |
R634A | 1 × 2,500u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer J 10X Buffer |
R009A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
KpnI |
R634G | 1 × 10,000u |
Resources
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