MULTI-CORE™ Buffer Pack

• Convenient and Economical: MULTI-CORE™ Buffer enables co-digestion of DNA with more than one enzyme in a single reaction. In most cases, only modest adjustments in the amount of enzyme used will ensure complete multiple digestions.

1X Restriction Enzyme Buffer Composition.

MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.

Notes:

1. For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
2. For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.
3. All restriction enzymes are supplied with 10mg/ml Acetylated BSA. Although BSA is not absolutely required for activity, it has been shown to enhance activity of many restriction enzymes. We recommend adding BSA to all restriction digests at a final concentration of 0.1mg/ml.