PstI

Cut Site:

C   TGCA^▼G
G_▲ACGT   C

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Notes:

1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
2. Ends generated by PstI can be directly ligated to ends generated by NsiI. Neither restriction site is regenerated in the ligation product.

Incubation Conditions: Buffer H. 37°C.

Source: Recombinant E. coli strain.

Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: