RsaI
- Capable of digesting DNA in 15 minutes or less
Catalog Number:
Choose a concentration
Size
Catalog Number: R6371
Cut Site:
GT▼AC
CA▲TG
Note: For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
Incubation Conditions: Buffer C. 37°C.
Source: Rhodopseudomonas sphaeroides.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-CORE™ |
|---|---|---|---|---|
| 75–100% | 75–100% | 100% | <10% | <10% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 113 | 83 | 11 | 3 | 19 | 3 |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer C 10X Buffer |
R003A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
RsaI |
R637A | 1 × 1,000u |
Resources
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