SgfI
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Genome qualified to ensure optimal performance in genomic analysis applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R7103
Cut Site:
GCG AT▼CGC
CGC▲TA GCG
Notes:
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- Sensitive to CpG methylation.
- SgfI is inactivated by freezing at –70°C.
- Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.
Incubation Conditions: Buffer C. 37°C.
Source: Streptomyces fulvissimis.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1% Thesit®, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-CORE™ |
|---|---|---|---|---|
| 25–50% | 25–50% | 100% | <10% | <10% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 0 | 1 | 0 | 0 | 0 | 0 |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer C 10X Buffer |
R003A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
SgfI |
R710B | 1 × 250u |
Resources
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