SmaI
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Genome qualified to ensure optimal performance in genomic analysis applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R6121
Catalog Number: R6125
Cut Site:
CCC▼GGG
GGG▲CCC
Note: XmaI is a neoschizomer of SmaI.
Incubation Conditions: Buffer J. 25°C. (SmaI exhibits 25–50% activity at 37°C.)
Source: Serratia marcescens.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | J | MULTI-CORE™ |
|---|---|---|---|---|---|
| <10% | <10% | <10% | <10% | 100% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 3 | 12 | 0 | 1 | 1 | 0 |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer J 10X Buffer |
R009A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
SmaI |
R612A | 1 × 1,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer J 10X Buffer |
R009A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
SmaI |
R612F | 1 × 5,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
Resources
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