TaqI
- Restriction endonuclease from Thermus aquaticus YTI
Catalog Number:
Choose a concentration
Size
Cut Site:
T▼CG A
A GC▲T
Note: Sensitive to overlapping dam methylation.
Incubation Conditions: Buffer E. 65°C. (TaqI exhibits <10% activity at 37°C.)
Source: Thermus aquaticus YTI.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | E | MULTI-CORE™ |
|---|---|---|---|---|---|
| 10–25% | 25–50% | 50–75% | 50–75% | 100% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 121 | 50 | 10 | 4 | 13 | 7 |
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
TaqI |
R615A | 1 × 1,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
TaqI |
R615E | 1 × 10,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
Resources
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