Glycogen-Glo™ Assay
Easy, Sensitive Glycogen Assay Kit
- Compatible with cell lysates and tissue homogenates
- In-well sample preparation protocol streamlines workflow
- Scalable to 384-well plates for HTS applications
Catalog Number:
Size
Catalog Number: J5051
Catalog Number: J5052
Efficient Glycogen Detection from Diverse Samples
The Glycogen-Glo™ Assay is a fast and sensitive method for detecting glycogen from various biological samples, such as cultured cells and tissue homogenates. Based on bioluminescent technology, the Glycogen-Glo™ Assay can detect subtle changes in glycogen synthesis, storage and breakdown. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation and spin columns. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.
How the Glycogen-Glo™ Assay Works
The Glycogen-Glo™ Assay utilizes a series of enzyme-coupled reactions. First, glycogen is digested into glucose using glucoamylase enzyme. The resulting glucose is measured using glucose dehydrogenase in conjunction with a bioluminescent NADH detection technology. The result is a light signal proportional to the starting glycogen concentration in the sample.
How to Determine Glycogen Concentration
Samples that contain both glycogen and glucose require two reactions to determine the glycogen concentration. One reaction is used to measure total glucose resulting from digested glycogen plus any glucose present in the sample. The second is used to measure any glucose that was present in the sample prior to digestion. The difference in signal between the two reactions represents the contribution from glycogen in the sample. The effective digestion of glycogen into glucose monomers (≥70%) simplifies data interpretation and calculations.
Simple Assay Protocol Saves Time
Accurately Detect the Smallest Changes in Glycogen
|
Sensitivity (S/B >5) |
0.6µg/ml |
|
|
Limit of Detection (S/N >3) |
20ng/ml |
|
|
Linear Range |
To 20µg/ml glycogen |
|
|
Maximum Assay Window (S/B) |
>150 |
|
Measure Glycogen Content of Cells in Culture
Cellular glycogen content varies greatly depending on cell type and growth conditions. The high sensitivity and wide linearity of the Glycogen-Glo™ Assay accommodate measurement of glycogen in cells, regardless of whether they have low or high glycogen levels. The data show analysis of intracellular glycogen content of three cell lines using the Glycogen-Glo™ Assay.
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Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
Reductase |
G884A | 1 × 55μl | 6mg/ml |
Reductase Substrate |
G885A | 1 × 55μl | |
Luciferin Detection Solution |
J135A | 1 × 5ml | |
NAD |
J136A | 1 × 30μl | |
Glucose |
J138A | 1 × 50μl | |
Glucose Dehydrogenase |
J140A | 1 × 200μl | |
Glucoamylase Buffer |
J403A | 1 × 3ml | |
Glucoamylase |
J404A | 1 × 25μl | |
Glycogen |
J405A | 1 × 50μl | |
0.3N HCl |
J406A | 1 × 15ml | |
Tris Buffer |
J407A | 1 × 15ml |
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
Reductase |
G884B | 1 × 275μl | 6mg/ml |
Reductase Substrate |
G885B | 1 × 275μl | |
Luciferin Detection Solution |
J135B | 1 × 50ml | |
NAD |
J136B | 1 × 275μl | |
Glucose |
J138A | 1 × 50μl | |
Glucose Dehydrogenase |
J140B | 2 × 1ml | |
Glucoamylase Buffer |
J403B | 1 × 30ml | |
Glucoamylase |
J404B | 1 × 150μl | |
Glycogen |
J405A | 1 × 50μl | |
0.3N HCl |
J406A | 1 × 15ml | |
Tris Buffer |
J407A | 1 × 15ml |
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
Resources
技術ポスター
その他
- Blog Article: Assays for Measuring Insulin Activity
- NAFLD & NASH Liver Disease Research Tools
- 糖尿病研究ツール
- Article: Bioluminescent Assay for the Quantification of Cellular Glycogen Levels
- Article: Small-molecule inhibition of glycogen synthase 1 for the treatment of Pompe disease and other glycogen storage disorders
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