Malate-Glo™ Assay
Easy, Sensitive L-Malate Assay Kit
- Compatible with cells, tissue and serum samples
- In-well sample preparation protocol streamlines workflow
- Scalable to 384-well plates for HTS applications
Catalog Number:
Size
Catalog Number: JE9100
Catalog Number: JE9200
Efficient Detection of Malate in Diverse Samples
The Malate-Glo™ Assay is a fast and sensitive method for detecting L-malate from various biological samples, such as cultured cells, tissue homogenates and serum. Based on bioluminescent technology, the Malate-Glo™ Assay can detect subtle changes in mitochondrial metabolism and the TCA cycle. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation and spin columns. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.
How the Malate-Glo™ Assay Works
Malate Dehydrogenase catalyzes the oxidation of malate with concomitant reduction of NAD+ to NADH. In the presence of NADH, Reductase enzymatically reduces the proluciferin Reductase Substrate to luciferin. Luciferin is detected using Ultra-Glo™ Recombinant Luciferase, and the amount of light produced is proportional to the amount of malate in the sample.
Simple Add-and-Read Protocol Saves Time
Accurately Detect the Smallest Changes in Malate
|
Sensitivity (S/B >5) |
400nM |
|
|
Limit of Detection (S/N >3) |
50nM |
|
|
Linear Range |
50nM to 25μM |
|
|
Maximum Assay Window (S/B) |
>200 |
|
Sensitive Quantitation of Malate in Cultured Cells
Cellular malate content varies depending on cell type, growth conditions and mitochondrial function. The Malate-Glo™ Assay, with its high sensitivity and wide linear detection range, measures malate levels in cell lysate samples using 390–25,000 cells per 50μl.
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Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
Malate Dehydrogenase |
J408A | 1 × 25μl | |
Malate, 10mM |
J409A | 1 × 50μl | |
Reductase |
G884A | 1 × 55μl | 6mg/ml |
Reductase Substrate |
G885A | 1 × 55μl | |
Luciferin Detection Solution |
J135A | 1 × 5ml | |
NAD |
J136B | 1 × 275μl | |
Neutralization Buffer |
J153A | 1 × 15ml | |
NADP+ |
J154B | 1 × 100μl | |
0.6N HCl |
J402A | 1 × 15ml |
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
Malate Dehydrogenase |
J408B | 1 × 250μl | |
Malate, 10mM |
J409A | 1 × 50μl | |
Reductase |
G884B | 1 × 275μl | 6mg/ml |
Reductase Substrate |
G885B | 1 × 275μl | |
Luciferin Detection Solution |
J135B | 1 × 50ml | |
NAD |
J136C | 1 × 1ml | |
Neutralization Buffer |
J153A | 1 × 15ml | |
NADP+ |
J154C | 1 × 500μl | |
0.6N HCl |
J402A | 1 × 15ml |
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
Resources
技術記事
技術ポスター
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