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Pyruvate-Glo™ Assay

Part Numbers: J4051, J4052

j4051-pyruvate-glo-assay-5-ml
j4052-pyruvate-glo-assay-50-ml
j4051-pyruvate-glo-assay-5-ml
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簡便、高感度なピルビン酸定量キット

  • 細胞、培地、血清など多様なサンプルに適応
  • 簡便なウェル内サンプル調製プロトコル
  • HTS用に384-ウェルプレートフォーマットへのスケールダウンも可能

Size

選択製品のカタログ番号: J4051

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Pyruvate-Glo™ Assay
5ml
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Rapid Pyruvate Detection from Diverse Samples

Pyruvate-Glo™ アッセイは、培養細胞、培地、血清など様々な生体試料中のピルビン酸を迅速かつ高感度に検出する方法です。生物発光技術を採用した Pyruvate-Glo™ アッセイは、解糖とミトコンドリア代謝により起こるわずかな変化を約75分で検出できます。その合理化されたサンプル調製プロトコールは、酸処理と中和をアッセイウエル内で直接行うため、細胞採取、遠心分離、スピンカラムが不要です。このアッセイは384ウェルフォーマットに適応可能で、多くのサンプルの迅速な分析を可能にします。

【毒劇物】対象品 ※SDSは本ページ下部より入手ください。

How the Pyruvate-Glo™ Assay Works

Diagram illustrating how the Pyruvate-Glo™ Assay works.

Pyruvate Oxidase catalyzes the reduction of pyruvate to acetyl phosphate, generating H2O2. In the presence of H2O2, the H2O2 Substrate is converted into luciferin. This reaction is detected by Ultra-Glo™ Recombinant Luciferase, which emits light proportionate to the amount of pyruvate in the sample.

Simple Assay Protocol Saves Time

Diagram illustrating the Pyruvate-Glo™ Assay protocol workflow.

Accurately Detect the Smallest Changes in Pyruvate

Graph showing the linear relationship between luminescence and pyruvate concentration.



Sensitivity (S/B >5)

1.56μM

Limit of Detection (S/N >3)

400nM

Linear Range

400nM to 50μM

Maximum Assay Window (S/B)

>150

Measure the Effect of Metabolic Inhibitors on Cellular Pyruvate Levels

The Pyruvate-Glo™ Assay can be used to monitor both intracellular and extracellular pyruvate in cell culture, including quickly assessing the metabolic effects of drug treatment.

Bar chart showing the effect on pyruvate levels after adding various drug compounds to K562 cells, as measured by the Pyruvate-Glo™ Assay.
Bar chart showing no decrease in cell viability after incubating K562 cells with various drugs.

Monitor metabolic effects of drug treatment with the  Pyruvate-Glo™ Assay. K562 cells in suspension were incubated with DMSO (negative control), 10μM GSK2837808 (lactate dehydrogenase A inhibitor), 10μM 7ACC2 (monocarboxylate transporter 1-4 [MCT1-4] inhibitor) or 10μM UK5099 (mitochondrial pyruvate carrier inhibitor) for one to two hours. Following the incubations, half the volume was analyzed using the Pyruvate-Glo™ Assay (Panel A), and half the volume was removed for viability analysis using CellTiter-Glo® Luminescent Cell Viability Assay (Panel B). As expected, pyruvate levels increased approximately twofold after a 2-hour incubation with UK5099 and 7ACC2 compared to the DMSO control. There was no decrease in viability during treatment when compared to the DMSO control. All data represent an average of three replicates.

Do you want to learn more about our cellular energy metabolism tools and the innovative technology that powers these assays?

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プロトコール

Complete Protocol

製品仕様

You are viewing: J4051 Change Configuration

選択製品の構成品内容

Item Part # Size

H2O2 Substrate, 10mM

 G882A 1 × 40μl

Signal Enhancer Solution

 G883A 1 × 100μl

H2O2 Substrate Dilution Buffer

 G922A 1 × 2ml

Luciferin Detection Solution

 J135A 1 × 5ml

Neutralization Buffer

 J153A 1 × 15ml

0.6N HCl

 J402A 1 × 15ml

Pyruvate Oxidase (POX)

 J416A 1 × 130μl

Pyruvate, 10mM

 J417A 1 × 50μl

d-Cysteine, 100X

 V251A 1 × 100μl

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

製品仕様

You are viewing: J4052 Change Configuration

選択製品の構成品内容

Item Part # Size

H2O2 Substrate, 10mM

 G882B 1 × 200μl

Signal Enhancer Solution

 G883B 1 × 500μl

H2O2 Substrate Dilution Buffer

 G922B 1 × 10ml

Luciferin Detection Solution

 J135B 1 × 50ml

Neutralization Buffer

 J153A 1 × 15ml

0.6N HCl

 J402A 1 × 15ml

Pyruvate Oxidase (POX)

 J416B 1 × 1300μl

Pyruvate, 10mM

 J417A 1 × 50μl

d-Cysteine, 100X

 V251B 1 × 500μl

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

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類似製品

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様々なサンプル種よりグルコースを簡便に検出

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様々なサンプル種より迅速に乳酸を検出する発光アッセイ

J5021, J5022

Malate-Glo™ Assay

Fast and sensitive method for detecting L-malate in various biological samples.

JE9100, JE9200

Metabolite-Glo™ Detection System

Bioluminescent NAD(P)H detection toolkit for metabolite detection in biological samples.

J9030, J9040

関連製品

RealTime-Glo™ MT Cell Viability Assay

72 時間まで培養細胞の生存性をモニタリングできる発光アッセイ

G9711, G9712, G9713

CellTiter-Fluor™ Cell Viability Assay

マルチアッセイに最適な細胞非溶解性の蛍光細胞生存試験

G6080, G6081, G6082

CellTiter-Glo® 2.0 Cell Viability Assay

試薬の安定性が向上したCellTiter-Glo®、ATP検出に基づく細胞増殖の定量が可能

G9241, G9242, G9243, MG1010

GloMax® Discover System

発光・蛍光・吸光度測定に対応した高性能マイクロプレートリーダー

GM3000

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