Plexor® HY System

Part Numbers: DC1001, DC1000, DC1500

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qPCRによるヒト総DNA濃度およびヒト男性DNA濃度の決定

  • ヒト総DNA濃度およびヒト男性DNA濃度を1回の反応で同時に測定
  • 約6.4pgの総DNAを検出可能
  • 偽陰性ならびに偽陽性検定用の内部PCRコントロール(IPC)と融解曲線機能

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選択製品のカタログ番号: DC1001

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Plexor® HY System
Plexor® HY System/200 reactions
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The Power to See It All in a Single Assay

Plexor® HY Systemは、ヒト総DNA濃度およびヒト男性DNA濃度を1回の反応で同時に測定するために特別に設計されたリアルタイムPCRアッセイです。キットには、PCR阻害剤が原因となる偽陰性を検定するための内部PCRコントロール(IPC)および目的の産物が増幅されていることを確認するための融解曲線機能が付加されています。
Plexor® HY Systemは、感度の高いマルチプレックスキットで、約6.4pgの総DNAを検出することができます。PCR増幅反応のセットアップは、室温で実施でき、自動化プラット フォームに適応します。
Plexor® HY Systemは、増幅とともに蛍光シグナルが減衰する新規な方法を採用しています。各標的の増幅には、プライ マー2つのみ(蛍光物質と修飾塩基を付加したプライマーと未標識のプライマー)を使用します。増幅が進むと、プライマー上の修飾塩基と相補する位置にクエンチャーが部位特異的に導入されることにより蛍光が減衰します。最終的なアッセイをデザインするための(内部標準を提供)、あるいはトラブルシューティングを効率よく行うための融解分析をPCR後に実施することができます。
Plexor® HY Systemは、Applied Biosystems 7500および7500 FAST real-time PCR systemsおよびStratagene Mx3005P®およびMx3000P® qPCR systemでの使用に最適化されています。他のqPCR装置で使用する際の情報についてはテクニカルサービス部までお問い合わせ下さい。Plexor® Analysis Softwareは、無償ダウンロードが行えます(www.promega. com/plexorhy/)。このユニークなソフトウエアはデータを迅速、簡便に考察し、レポートの作成を容易にします。リプリケートサンプルでは自動的に平均値が得られ、鋳型量を算出して、最適なSTR増幅条件に適したDNA量を提示します。


  • 常染色体およびY染色体DNAを同時に定量: バラツキ、所要時間が少なく、より精度の高いデータが得られます
  • 6.4pgのDNAでも安定に検出: Plexor®HYで検出できないサンプルは、STRsystemで検出不能
  • 内部陽性対照および融解曲線分析: 偽陰性および偽陽性を監視するため、データの信頼性が向上

さらに知りたい方は、プロトコール&ガイド(英語)をご覧ください。


  1. Johnson, S.C. et al. (2004) Nucl. Acids Res. 32, 1937–41.
  2. Frackman, S. et al. (2005) Promega Notes 90, 2–4.
  3. Sherrill, C.B. et al. (2004) J. Am. Chem. Soc. 126, 4550–6.
  4. Moser, M.J. et al. (2005) Antimicrob. Agents Chemother. 49, 3334–40.
  5. Krenke, B. and Knox, C. (2007) Forensic Magazine.
  6. Noreault-Conti, T. and Buel, E. (2007) Profiles in DNA 10(1), 3–5.
  7. Katz, D.E. (2007) Profiles in DNA 10(1), 6–8.
  8. Krenke, B. (2005) Profiles in DNA 8(2), 6–7.
  9. Krenke, B. et al. (2005) Profiles in DNA 8(1), 3–5.

ForensicGrade

プロメガの法医学関連製品は ISO 18385 に準拠して製造されています。これにより法医学的な解析を目的とする生体サンプルの採取、保存、分析を行うための製品にヒトDNAが混入するリスクが最小限に抑えられます。 詳細についてはこちら

If There is DNA to Detect, Plexor® HY System Will Show It

The system works by measuring a reduction in fluorescent signal during amplification. Amplification of each target uses only two primers, one of which contains both a fluorescent tag and a modified base. As amplification proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent quencher opposite the complementary modified base. The quencher is in close proximity to a fluorescent dye located on the end of the primer, resulting in a reduction of fluorescent signal. After PCR, a melt-curve analysis can be performed to provide an internal control for the final assay design or to expedite troubleshooting.

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Want to Automate?

Save time and improve precision by automating your DNA quantification setup. The PowerQuant® and Plexor® HY Systems can be automated on the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan), or MicroLab STAR (Hamilton) liquid handlers. Additionally, import the quantification data into the STR Normalization Manager Software to automate your PCR amplification setup.

Automate your DNA Quantification Setup

Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.

Hardware
Product Cat.#
Four-Position Tube Holder
Plate Clamp 96
Plate Stand

製品仕様

You are viewing: DC1001 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration Available Separately

Plexor® HY Male Genomic DNA Standard

DD149A 1 × 150μl 50ng/μl

Plexor® HY 20X Primer/IPC Mix

DK716A 1 × 200μl

Plexor® HY 2X Master Mix

DP120A 2 × 1ml

Water, Amplification Grade

DW099A 2 × 1,250μl View Product

分析証明書

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使用制限

Not For Medical Diagnostic Use.

保存条件

BB

パテントおよび免責条項

CAL Fluor® technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc., for research and development and forensic and paternity testing. These products are sold for use by the end-user only and may not be resold, distributed or repackaged.

The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Pat. Nos. 7,422,850, 7,517,651 and 7,541,147 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Business Development, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.

製品仕様

You are viewing: DC1000 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration Available Separately

Plexor® HY Male Genomic DNA Standard

DD149A 3 × 150μl 50ng/μl

Plexor® HY 20X Primer/IPC Mix

DK716A 4 × 200μl

Plexor® HY 2X Master Mix

DP120A 8 × 1ml

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

BB

パテントおよび免責条項

CAL Fluor® technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc., for research and development and forensic and paternity testing. These products are sold for use by the end-user only and may not be resold, distributed or repackaged.

The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Pat. Nos. 7,422,850, 7,517,651 and 7,541,147 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Business Development, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.

製品仕様

You are viewing: DC1500 Change Configuration

選択製品の構成品内容

Item Part # Size

Plexor® Concentrated Fluorescein Calibrator

DS101A 1 × 50μl

Plexor® Concentrated CAL Fluor® Orange 560 Calibrator

DS102A 1 × 50μl

Plexor® Concentrated CAL Fluor® Red 610 Calibrator

DS103A 1 × 50μl

Plexor® Concentrated IC5 Calibrator

DS104A 1 × 50μl

Plexor® 1X Calibration Buffer

DS105A 1 × 11ml

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

BB

パテントおよび免責条項

CAL Fluor® technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc., for research and development and forensic and paternity testing. These products are sold for use by the end-user only and may not be resold, distributed or repackaged.

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