Bone DNA Extraction Kit
Rapidly Process Bone and Teeth Samples
- Quick preprocessing of bone powder using Demineralization Buffer
- Compatible with manual and Maxwell® DNA IQ™ chemistry
- Yields DNA free of inhibitors and compatible with downstream amplification reactions
- Note: The Bone DNA Extraction Kit (Cat.# DC6051) replaces the custom version of the Bone DNA Extraction Kit (Cat.# AX6780). No changes have been made to the product composition, and therefore, no performance changes are expected during the transition of the product from the custom to catalog version.
Catalog Number:
Size
Catalog Number: DC6051
骨からDNA抽出を行うための迅速な処理
Extraction of DNA from challenging bone samples. Femur bone samples dated from 1939–1945 (World War II) and 1870–1880 (Deadwood) were used with the Bone DNA Extraction Kit, Custom, followed by purification using DNA IQ™ chemistry on the Maxwell® Instrument. Autosomal DNA concentration, as determined by PowerQuant® System, was plotted as vertical bars. The normalized DNA or maximum template amount was used for amplification using the PowerPlex® Fusion System, and the number of alleles detected are shown.
行方不明、大規模災害、人類学的発掘調査といった特定の場合において、しばしば骨や歯は身元を確認するための唯一のサンプルとなります。さらにこれらのサンプルは環境によるダメージを受けていたり精製途中に環境由来の阻害物質が共抽出され、STRによる型判定の有用性が制限されます。阻害物質を含まないDNAを精製することは、このようなサンプルからの有用なプロファイル取得を決定づける極めて重要な第一ステップです。
短い前処理プロトコールの後、DNA IQ™試薬でDNAを精製することで得られる阻害物質フリーのDNAは、ヒトおよびヒト男性DNAの定量用試薬であるPowerQuant®もしくはPlexor® HY System、そしてSTRプロファイル生成用試薬であるPowerPlex® STR Systemに使用できます。
SDS については こちら
Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
使用方法の概要
1.5mlのマイクロチューブに入れた骨粉に400µlのpreprocessing bufferを加え、1000rpmの震盪機にセットして56ºC、2.5時間インキュベートする。その後13,000 × g で5分間遠心し、上清をLysis bufferと還元剤が入った1.5mlのマイクロチューブに移す。この時点で、この前処理サンプルはDNA IQ™ （手動、Maxwell®のどちらでも）で精製する準備が完了する。処理済みの骨粉ペレットが入った1.5mlのマイクロチューブは廃棄して良い。精製の後、DNAは50µlのelution bufferに溶出され、定量試薬であるPowerQuant® SystemやSTRプロファイル生成用試薬であるPowerPlex® Systemに使用できる。
Bone DNA Extraction Kit, Customをラボのワークフローに組み込むことで、貴重なサンプルから迅速でコストパフォーマンスの高いDNA抽出を最小限の作業時間で実施できます。
Ideal for STR Amplification or Massively Parallel Sequencing Workflows
Due to the advantages that massively parallel sequencing (MPS) offers when working with challenging samples, numerous casework laboratories have adopted MPS as a technique when typical STR amplification fails to yield a usable profile. DNA purified with the Bone DNA Extraction Kit can be used in MPS applications. A workflow is outlined below as an example.
Twenty-five-year-old tooth samples from siblings were processed using the Bone DNA Extraction Kit and Maxwell® FSC DNA IQ™ Casework Kit. The extracted DNA was subsequently used in a massively parallel sequencing application as outlined above. Results from the PowerQuant® quantitation assay and library coverage details are shown for duplicates of the two samples. Sequencing results yielded 13 variant calls from each sibling sample that were concordant with each other and were assigned to the H2c haplogroup. Sequencing was performed with the prototype version of the PowerSeq® Whole Mito System.
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Demineralization Buffer |
D678A | 1 × 50ml | |
|
Proteinase K (PK) Solution |
MC500B | 1 × 4ml | View Product |
|
1-Thioglycerol |
A208B | 1 × 900μl | |
|
Lysis Buffer |
A826B | 1 × 150ml | View Product |
Related Products
関連製品
Maxwell® FSC Instrument
Benchtop instrument for automated DNA extraction from casework samples.
AS4600
Maxwell® FSC DNA IQ™ Casework Kit
Extract DNA from 1–16 casework samples using the Maxwell® FSC Instrument.
AS1550
PowerQuant® System
5-color, 4-target probe-based qPCR assay.
PQ5002, PQ5008, DS1221
PowerPlex® Fusion System
Co-amplification and fluorescent detection of 24 loci.
DC2402, DC2408
