Casework Direct System
Rapidly Process Sexual Assault Evidence and Low Abundance DNA Samples
- No-wash protocol maximizes the chance of recovering DNA from trace samples
- Quickly generates lysates that are compatible with PowerQuant®, Plexor® HY and PowerPlex® Systems
- Useful for Y-screening and selection of evidentiary samples for STR testing
- Note: The Casework Direct System (Cat.# DC4560, DC4561) replaces the custom version of the Casework Direct Kit, Custom (Cat.# AX4560). No changes have been made to the product composition and, therefore, no performance changes are expected during the migration of the product from the custom to catalog version.
Catalog Number:
Size
Catalog Number: DC4560
Catalog Number: DC4561
Rapidly Process Challenging Sample Types
The sheer volume of property crime and sexual assault evidentiary samples submitted to forensic laboratories has compelled many laboratories to actively seek out better solutions for processing these challenging sample types. The Casework Direct System is designed to rapidly process swabs from casework samples or cuttings of sexual assault swabs and stained clothing. (Read more in this white paper—download article.)
Unlike competing kits, lysates generated with the Casework Direct System are compatible with our quantification and STR amplification product lines. These lysates may be amplified with the PowerQuant® or Plexor® HY Systems to screen sexual assault samples for male DNA and to normalize human template for STR amplification with one of the PowerPlex® STR Systems.
When used with the PowerQuant® System, valuable workflow information such as the presence/absence of male DNA, degradation or potential PCR inhibition is provided.
Learn more about how the Casework Direct System and the PowerQuant® System can help improve processing efficiency for sexual assault evidence kits (SAEK).
プロメガの法医学関連製品は ISO 18385 に準拠して製造されています。これにより法医学的な解析を目的とする生体サンプルの採取、保存、分析を行うための製品にヒトDNAが混入するリスクが最小限に抑えられます。 詳細についてはこちら
使用方法の概要
Add 100–400µl of Casework Direct Solution along with a swab or cutting in the CW Spin Basket assembly, vortex for 5–10 seconds and incubate at 70°C for 30 minutes. Vortex again for 5–10 seconds, and centrifuge at room temperature for 5 minutes at maximum speed in a microcentrifuge to collect the lysate in the 1.5ml microcentrifuge tube. Carefully remove the CW Spin Basket containing the swab or cutting, and discard the spin basket. Quantify 2µl of the lysate using PowerQuant® System or Plexor® HY System. Amplify up to 15µl of the lysate using a PowerPlex® System.
Y-Screeningアプリケーション
性的暴行サンプルの従来の血清学的スクリーニングは手間がかかり、増幅による男性DNAの検出と比較すると感度が限られていました。Casework Directのプロトコールには洗浄ステップがなく、研究室のコスト（労力と試薬）ならびにDNA抽出ステップで見られるDNAのロスを低減できます。さらに、従来のスクリーニング結果からではどのようなDNAプロファイルが得られるか予測することは困難でした。従来の血清学的方法に関連した限界と課題を認識し、新しい SWGDAM recommendations は血清学の前にDNA解析を実施しCODIS適格プロファイルを得る機会を最大化する「Direct to DNA」アプローチを指示しています。
Detection of male DNA in semen dilution series. Dilutions of human semen were spotted on cotton swabs. 400µl of Casework Direct Solution was added to the swab placed in a spin basket assembly, incubated at 70°C for 30 minutes and centrifuged at max speed for 5 min to recover the lysate. 2µl of the lysate was used to quantify the amount of human amplifiable DNA using PowerQuant® System and 0.5ng or maximum volume of lysate was used for STR amplification using PowerPlex® Fusion and PowerPlex® Y23 System. The amplified products were electrophoresed on 3130xl using POP4 with 3kV, 5 sec injection and analyzed on GeneMapper® IDv3.2 with an analysis threshold of 50 RFU. The percentage of alleles called is shown on the left axis and the corresponding template amount on the right y-axis. Less than 0.5ng template amount was available for 1:100 and 1:1000 dilution samples. Profiles were called at all dilution except 1:1000 for PowerPlex® Fusion and Y23.
ケースワーク "タッチ" DNAサンプル
この数年間、法執行機関では凶悪犯罪の証拠提出が増加し、「タッチDNA」ーDNAを含むごく小さな皮膚の細胞が何か（例えば車のハンドル）に触れた際に自然にはがれおちたものー の証拠分析に研究室の多くのリソースを割かなくてはならなくなっています。DNAが凶悪犯罪の現場で見つかると、研究室ではDNAデータベースであるCODISに登録されている既知の犯罪者のサンプルと照合することができます。
Casework Direct Kit, Customを研究室のワークフローに統合することで、貴重で微量なサンプルから高品質なSTRプロファイルが最小限のハンズオンタイムで得られます。PowerQuant® Systemと併用すると、男性DNA分解の有無やPCR阻害の可能性など有益な情報が取得できます。いくつかのモックケースワークサンプルを用いてSTR解析を実施した結果を以下に示します。
Casework Direct Solution for trace DNA samples. Cotton swabs were used to collect trace DNA samples from steering wheel, computer mouse and cell phone surfaces. 400µl of Casework Direct Solution was added to the swab placed in a spin basket assembly, incubated at 70ºC for 30 minutes and centrifuged at 16,000 × g for 5 minutes to recover the lysate. 2µl of the lysate was used to quantify the amount of human amplifiable DNA using PowerQuant® System and 0.5ng or maximum volume of lysate was used for STR amplification using PowerPlex® Fusion and PowerPlex® Y23 Systems. The amplified products were electrophoresed on the Applied Biosystems® 3500xL Genetic Analyzer using POP4 with 3kV, 5 second injection and analyzed on GeneMapper® IDv3.2 with an analysis threshold of 50 RFU.
Additional Information
Rapid Casework Sample Processing
This white paper describes the developmental validation of the Casework Direct Kit, Custom, for rapid processing of samples ranging from swabs to trace DNA samples.
Rapid, Cost-Effective Processing of Property Crime Samples
This brochure provides an overview on how to use the Casework Direct System to generate high-quality STR profiles from precious, low-abundance samples.
Improve SAEK Processing Efficiency
This brochure highlights using the Casework Direct System for a "Direct-to-DNA" approach, to maximize the chances of obtaining CODIS-eligible profiles.
Stability of Lysates
This white paper presents the results of long-term storage testing at 4°C and 28°C, as well as freeze-thaw studies, of lysates prepared with the Casework Direct System.
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
| 1-Thioglycerol | A208A | 1 × 75μl | |
| 5X AmpSolution™ Reagent | DM123A | 1 × 500μl | View Product |
Casework Direct Reagent |
DM456A | 1 × 100ml | |
|
Water, Amplification Grade |
DW099A | 5 × 1,250μl | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
| 1-Thioglycerol | A208A | 1 × 75μl | |
| 5X AmpSolution™ Reagent | DM123A | 1 × 500μl | View Product |
Casework Direct Reagent |
DM456B | 1 × 10ml | |
|
Water, Amplification Grade |
DW099A | 2 × 1,250μl | View Product |
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