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PowerSeq™ CRM Nested System, Custom

Part Numbers: AX5810

ミトコンドリアDNAコントロール領域のNGS用ライブラリー作製

  • 1反応でミトコンドリアHVI、HVII、HVIIIコントロール領域の次世代シーケンシング
  • Nested-PCR法によりライブラリー調製にかかる実験ステップと時間を大幅低減
  • 劣化したDNAサンプルでもより良い結果が得られるよう、144-237bpの小さなアンプリコン
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Size

選択製品のカタログ番号: AX5810

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PowerSeq™ CRM Nested System, Custom
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次世代シーケンスであれば、従来のシーケンシング方法よりも高感度でハイスループットなワークフローを用いたミトコンドリアDNA（mtDNA）解析が可能です。高いシーケンスカバレッジやデジタルリードカウントにより混合サンプルのデコンボリューションとヘテロプラスミーの解像度が向上しました。さらにミトコンドリアコントロール領域のシーケンシングに小さなアンプリコンを用いることで、分解されたサンプルからのシーケンシング結果がより良いものとなっています。

PowerSeq™ CRM Nested System, Customミトコンドリアゲノムのコントロール領域をカバーする10個のアンプリコンを1反応で1度に作製できます。分解されたサンプルからでも最適な結果を得るため、増幅のターゲット領域は144-237bpのサイズとなるようデザインされています。本システムではNested-PCR法を採用し、ライブラリー調製にかかる実験ステップと時間が大幅に少なくなりました。1回のPCR反応でターゲットアンプリコンとインデックスアダプターの付加を同時に行うプロトコールです。

NDIS Approved

The PowerSeq® CRM Nested System is now approved for use in laboratories that generate DNA profiles for submission to the National DNA Index System (NDIS).

For more information, see: Brandhagen, M.D. et al. (2019) Validation of NGS for mitochondrial DNA casework at the FBI Laboratory. Forensic Sci. Int. Genet. 44, 102151.

Read Article

Nested-PCRでワークフローをシンプルに

PowerSeq™ CRM Nested System, Customはターゲットシーケンスを増幅するためのプライマーと、MiSeq®でのシーケンシングに必要なインデックスとアダプター配列を含むプライマーの組み合わせています。このワークフローにより時間短縮、サンプルロスとデータのバラつきが低減され、ライブラリー調製がシンプルになりました。

PowerSeq™ CRM Nested System, Custom, simplified workflow

Adapted from Lange et al. (2014) BMC Genomics 15, 63.

Balanced Representation of Pooled Libraries

Representative balanced profile of PowerSeq® CRM Nested System, Custom.

Representative balanced profile of PowerSeq® CRM Nested System, Custom. 2800M Control DNA (500pg; n=4) was amplified with the PowerSeq® CRM Nested System. A pool of 16 samples was sequenced simultaneously using MiSeq® Reagent Kit v3. Balance data was analyzed with ExactID® (Battelle Memorial Institute).

プロトコール

Complete Protocol

製品仕様

選択製品の構成品内容

Item Part # Size Concentration Available Separately

PowerSeq® CRM Nested 10X Primer Pair Mix

 AX579A 1 × 250μl

PowerSeq® Nested System Index Primer 1 D701

 AX580A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D702

 AX582A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D703

 AX583A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D704

 AX584A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D705

 AX585A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D706

 AX586A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D707

 AX587A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D708

 AX588A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D709

 AX589A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D710

 AX590A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D711

 AX591A 1 × 75μl

PowerSeq® Nested System Index Primer 1 D712

 AX592A 1 × 75μl

PowerSeq® Nested System Index Primer 2 D501

 AX593A 1 × 75μl

PowerSeq® Nested System Index Primer 2 D502

 AX594A 1 × 75μl

PowerSeq® Nested System Index Primer 2 D503

 AX595A 1 × 75μl

PowerSeq® Nested System Index Primer 2 D504

 AX596A 1 × 75μl

PowerSeq® Nested System Index Primer 2 D505

 AX597A 1 × 75μl

PowerSeq® Nested System Index Primer 2 D506

 AX598A 1 × 75μl

PowerSeq® Nested System Index Primer 2 D507

 AX599A 1 × 75μl

PowerSeq® Nested System Index Primer 2 D508

 AX600A 1 × 75μl

2800M Control DNA

 DD710A 1 × 25μl 10ng/μl View Product

PowerSeq® 5X Master Mix

 DP116A 1 × 500μl

Water, Amplification Grade

 DW099A 2 × 1,250μl View Product

Proteinase K

 V302A 1 × 10mg View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

Not For Medical Diagnostic Use.

保存条件

EE

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