ProNex NGS Library Quant Kit

Part Numbers: PS5000

PS5000_PowerSeq Quant MS_3
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NGSライブラリの正確で再現性の高い定量

  • インタクトで増幅可能なライブラリ濃度を測定
  • 生産性を最大にするBRYT Green®色素ベースのqPCRシステム
  • Illuminaライブラリのマルチプレックスプーリングに最適

Size

選択製品のカタログ番号: PS5000

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ProNex NGS Library Quant Kit
500 reactions
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Consistent Quantification for MPS Libraries

The PowerSeq® Quant MS System is a qPCR-based system designed for library quantification within the MPS workflow by directly measuring the number of DNA molecules present in your libraries. Accurate quantification of a library determines the optimal amount of adapter ligated fragments for cluster generation and ensures even representation of each library on the chip.

Balanced Representation of Pooled Libraries

Use of the PowerSeq® Quant MS System ensures that your libraries are evenly represented and precisely quantified on the MiSeq® flowcell. This results in more consistent and reproducible sequencing results.

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Thirty-two libraries were quantified using the PowerSeq® Quant MS System. The resulting values were used to normalize loading of samples into a final sequencing pool. The percent of reads identified for each sample from the total reads generated in the sequencing run demonstrates how evenly each sample was represented on the MiSeq® flowcell in relation to all libraries sequenced.

製品仕様

選択製品の構成品内容

Item Part # Size Concentration Available Separately

PowerSeq® Quant 2X Master Mix

DP115A 5 × 1ml

PowerSeq® Quant MS 10X Primer Mix

DK803A 1 × 1ml

PowerSeq® Quant MS DNA Standard

DD104A 1 × 200μl 200pM

PowerSeq® Quant MS 1X Dilution Buffer

DM146A 2 × 150ml

Water, Amplification Grade

DW099A 2 × 1,250μl View Product

分析証明書

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使用制限

Not For Medical Diagnostic Use.

保存条件

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パテントおよび免責条項

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

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