NanoLuc® Labeling System

Part Numbers: VB1500

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ELISA で使用する抗体、その他タンパク質の標識に

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選択製品のカタログ番号: VB1500

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NanoLuc® Labeling System
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生物発光 ELISA のための HaloTag®-NanoLuc® による標識

NanoLuc® Labeling Systemは、小型(19.1kDa)で安定したレポーター酵素であり、明るい発光シグナルを生成する NanoLuc®ルシフェラーゼをベースとしています。NanoLuc® Labeling Systemでは、抗体をNanoLucで化学的に標識し、直接法、競合法、間接法の免疫測定に使用することができます。

抗体をNanoLuc®ルシフェラーゼで標識するために HaloTag®テクノロジーが用いられています。HaloTagは、生理的条件下でそのリガンド(HaloTag® Ligand)と共有結合する融合タンパク質で、抗体の標識を含む様々な用途に使用されています。アミン反応性の HaloTag® Succinimidyl Ester (O4) Ligand (509Da) と抗体上のリジンアミノ酸の一級アミンとの反応を含む2ステップのプロセスにより標識されます。この反応のために、抗体はタンパク質保存剤を含まないアミンフリーのバッファーに入れておく必要があります。

NanoLuc® ルシフェラーゼによる抗体の標識

How to label antibodies with the Nanoluc luciferase labeling system

 NanoLuc-HaloTag による抗体の標識プロセスは他のタンパク質標識にも応用できます。

NanoLuc 標識抗体を用いた ELISA のデータ

ELISA data using Nanoluc luciferase-labeled antibody

NanoLuc®ルシフェラーゼで標識した抗EGFR抗体を用いた直接ELISA(青丸)と、ビオチンで標識した抗EGFR抗体とストレプトアビジンで標識したNanoLuc®ルシフェラーゼを組み合わせた間接ELISA(赤四角)。

Bioluminescence Resource Center

Learn more about how luminescence works, what makes it different from fluorescence and how you can use luminescence to fuel your own research.

製品仕様

選択製品の構成品内容

Item Part # Size Concentration

IGEPAL® CA-630

VB141A 1 × 100μl

HaloTag® Ligand

VB125A 1 × 20μl 20mM

NanoLuc-HaloTag

VB131A 1 × 350μl 20μM

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

NanoLuc® Labeling System Limited Use Label License

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only and may link NanoLuc®-HaloTag® fusion proteins to researcher-supplied moieties. No transfer or commercial use of this product or derivatives is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; and (2) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Notwithstanding the foregoing, researcher may use this product or derivatives in provision of services, information or data to third parties in exchange for consideration, provided that researcher does not transfer the product or derivatives. No other use of this product or its derivatives is authorized without the prior express written consent of Promega.

In addition, researcher must either:
(i) use Lumit®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(ii) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 9,540,402, European Pat. No. 2369006, Japanese Pat. No. 474868 and other patents.

U.S. Pat. Nos. 9,873,866 and 10,246,690; European Pat. Nos. 2341134, 2374875, 2395078, 2492342 and 2502990; Japanese Pat. Nos. 5680302 and 5840117 and other patents.

U.S. Pat. No. 10,233,485 and 10,774,364; European Pat. Nos. 2990478 and 3498710; Japanese Pat. No. 6539689; and other patents and patents pending.

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