Lumit® Active IL-18 (Human) Immunoassay
No-Wash Immunoassay for Active IL-18
- Specific detection of mature/active IL-18; does not recognize pro-IL-18
- Fast add-and-read protocol
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W1910
Catalog Number: W1911
Catalog Number: W1912
Catalog Number: W191A-C
リン酸化または総タンパク質の高速定量
Lumit™ Immunoassay Cellular System は、細胞溶解液に含まれる標的分子を直接測定可能な洗浄のいらない生物発光イムノアッセイです。このアッセイは、適切な一次抗体（お客様提供）が利用可能であれば、あらゆるリン酸化タンパク質、総タンパク質あるいは低分子を検出できるように適合させることができます。
ご参考いただくために、注目度の高いターゲットについて市販の一次抗体を用いて Lumit™ イムノアッセイの評価を行いました。以下のアプリケーションノートには、試用した各一次抗体用のプロトコル、サンプルデータ、最適化条件、および注文情報が記載されされています。
How the Lumit® IL-18 Immunoassay Works
Lumit® Flex technology is a luminescent structural complementation system composed of two small peptide tags (Peptide α and Peptide β) and a reagent-based polypeptide (Lumit® Flex Detection Protein). In the Lumit® Active IL-18 Immunoassay, a monoclonal anti–human IL-18 antibody and human IL-18 binding protein (IL-18BP) are labeled with Peptide α and Peptide β, respectively. In the presence of active IL-18, the two peptides are brought into proximity. When Lumit® Detection Reagent B (containing the Flex Detection Protein) is added, a functional luciferase is reconstituted and produces a bright luminescent signal in the presence of Lumit® Substrate. The signal is directly proportional to active IL-18 levels.
The assay specifically detects active IL-18 and does not recognize pro-IL-18 or IL-18 bound to endogenous IL-18BP.
For proper assay performance, hIL-18BP-β must be incubated with cells during treatment to capture active IL-18 immediately as it is released from cells. This is because active IL-18 is unstable in cell culture media. To see data demonstrating the instability of active IL-18, review this scientific poster.
Simple One-Plate Protocol Requires No Wash Steps
Lumit™ イムノアッセイ vs. ウェスタンブロッティング
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Specification |
Lumit® IL-18 Immunoassay |
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Dynamic Range |
7.8pg/ml–5,714pg/ml |
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Limit of Detection (LOD) |
1.9pg/ml (3 SD above background) |
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Minimal Detectable Dose (MDD) |
1.6pg/ml (2 SD above background) |
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Assay Time |
70 minutes |
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Sample Type |
Cell culture supernatants |
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SD=Standard Deviation
Featured Webinar
Using Lumit® Technology to Address Inflammasome-Mediated Cytokine Release
In this webinar, Promega Sr. Research Scientist, Dr. Martha O’Brien, describes developing tools to measure inflammasome activation, including the Lumit® Active IL-18 Immunoassay and Lumit® IL-1β Immunoassay. Dr. O’Brien shows how Lumit® Assays can be multiplexed with other inflammasome assays to identify and rank the potency of inflammasome inhibitors.
Detect IL-18 Release Following Inflammasome Activation
The Lumit® Active IL-18 (Human) Immunoassay was used to quantitate secretion of active IL-18 from THP-1 cells following treatment. Active IL-18 is found in cell culture media samples following treatment with either nigericin or nigericin + LPS but not in untreated cells or cells treated with LPS alone. The amount of active IL-18 secreted was calculated using the standard curve. Data shown is an average of 3 replicates; error bars represent standard deviation.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml |
Lumit® Flex Detection Protein |
VB720A | 1 × 450μl |
hIL-18BP-Peptide β, 1000X |
W192A | 1 × 20μl |
Anti-hIL-18 mAb-Peptide α, 1000X |
W193A | 1 × 20μl |
Lumit® Human Active IL-18 Standard |
W191A-C | 1 × 25μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml |
Lumit® Flex Detection Protein |
VB720B | 1 × 4.5ml |
hIL-18BP-Peptide β, 1000X |
W192B | 1 × 200μl |
Anti-hIL-18 mAb-Peptide α, 1000X |
W193B | 1 × 200μl |
Lumit® Human Active IL-18 Standard |
W191A-C | 1 × 25μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml |
Lumit® Flex Detection Protein |
VB720A | 5 × 450μl |
hIL-18BP-Peptide β, 1000X |
W192A | 5 × 20μl |
Anti-hIL-18 mAb-Peptide α, 1000X |
W193A | 5 × 20μl |
Lumit® Human Active IL-18 Standard |
W191A-C | 1 × 25μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Lumit® Human Active IL-18 Standard |
W191A-C | 1 × 25μl |
Resources
技術記事
- Lumit® Cytokine Assays: Interpolating Data with the GloMax® Discover
- Easier Detection of Inflammasome Activation
- High-Throughput Cytokine Detection with Lumit® Technology
- A Comprehensive Toolkit for NLRP3 Inflammasome Drug Discovery
- Immunoassay Guide
- White Paper: Lumit® Immunoassays: A Rapid and Sensitive Method for Analyte Detection
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
技術ポスター
- Lumit® Immunoassays: Bioluminescent, Sensitive, and Homogeneous Analyte Detection Using Labeled Antibodies
- Rapid and Sensitive Determination of Cytokine Release from Cells Without Sample Transfer
- A Lumit® Human IL-18 Assay Specifically Measures Active IL-18 Release in Cell Culture
- SLAS 2024 Lumit Cytokine Assay Automation for HTS
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