Lumit® Immunoassay Cellular Systems
No-Wash, One-Plate Immunoassay for Cellular Analytes
- New Lumit® Lysis Buffer II included! Offers enhanced performance across a wide range of cellular targets
- New Application Notes available for more validated targets
- Set 1: Lumit® Anti-Mouse Ab-LgBiT/Lumit® Anti-Rabbit Ab-SmBiT
- Set 2: Lumit® Anti-Mouse Ab-SmBiT/Lumit® Anti-Rabbit Ab-LgBiT
- Starter Kit: Includes all four Set 1 and Set 2 Lumit® Secondary Antibodies; primary antibodies supplied by the user
Catalog Number:
Choose a System
Size
Catalog Number: W1201
Catalog Number: W1202
Catalog Number: W1203
Catalog Number: W1331
Catalog Number: W1332
Catalog Number: W1333
Catalog Number: W1220
リン酸化または総タンパク質の高速定量
Lumit® Immunoassay Cellular System は、細胞溶解液中の標的分子を直接測定可能な洗浄のいらない生物発光イムノアッセイです。このアッセイは、適切な一次抗体（お客様提供）が利用可能であれば、あらゆるタンパク質やリン酸化タンパク質あるいは低分子を検出可能です。
新しい溶解バッファー（Lumit® Lysis Buffer II）を開発し、Lumit® イムノアッセイ細胞システムで解析可能な細胞ターゲットの範囲が拡大されました（下表参照）。
また、pERK, pAKT, pSTAT3, pIkBα, pBTKなどの主要なシグナル伝達ノードを対象として、抗体セットを含む プレデザインキット が early access製品 としてご利用いただけます。
お客様の時間を節約するために、一般的ないくつかのターゲットについて市販の一次抗体を用いて評価しています。以下のアプリケーションノート集には、各一次抗体のプロトコール、データ例、最適化条件、注文方法などが記載されています。
Cellular Pathway Analysis Series
*New Lysis Protocol using Lumit® Lysis Buffer II.
| Human Cellular Target | Signaling Pathway | Lumit® Immunoassay Cellular System (Cat.#) |
Application Note / Complete Kit |
|---|---|---|---|
| Phospho-4E-BP1 (Ser 65) | mTor | Set 2 (W1331) | App Note |
 AKT |
PI3K/mTOR/AKT | Set 1 (W1201) | App Note |
| Phospho-AKT (Ser 473) | PI3K/mTOR/AKT | Set 1 (W1201) | App Note*/ Complete Kit |
| BCL6 | B-cell signaling | Set 1 (W1201) | App Note |
| β-catenin (Human) | WNT | Set 2 (W1331) | App Note |
| Phospho-β-catenin (Thr 41/Ser 45) | WNT | Set 2 (W1331) | App Note |
| Burton's Tyrosine Kinase (BTK) |
B-cell Receptor (BCR) | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-BTK (Tyr 223) | B-cell Receptor (BCR) | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-CHK1 (Ser 317) |
DDR Pathway | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-c-Jun (Ser 63) | JNK | Set 1 (W1201) | App Note |
| Phospho-c-MET (Tyr 1234/1235) |
c-MET | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-c-MET (Tyr 1349) |
c-MET | Set 1 (W1201) | App Note |
| CREB | PKA/CREB | Set 1 (W1201) | App Note |
| Phospho-CREB (Ser 133) | PKA/CREB | Set 1 (W1201) | App Note |
| EGFR |
EGFR | Set 1 (W1201) | Complete Kit |
| Phospho-EGFR (Tyr 1068) |
EGFR | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-EGFR (Tyr 1173) |
EGFR | Set 1 (W1201) | App Note |
| ER (Estrogen Receptor) | Estrogen | Set 1 (W1201) | App Note |
| Phospho-ERK1 (Thr 202) | ERK | Set 1 (W1201) | App Note*/ Complete Kit |
| Phospho-GSK1-3β (Ser 9) |
GSK-3β | Set 1 (W1201) | App Note |
| Phospho-H2AX |
DDR Pathway | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-HER2 (Tyr 1196) |
HER2 | Set 1 (W1201) | App Note |
| Phospho-HER2 (Tyr 1221/1222) |
HER2 | Set 1 (W1201) | App Note |
| IκBα | NF-κB | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-IκBα (Ser 32) | NF-κB | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-JNK (Thr 183/Tyr 185) | JNK | Set 1 (W1201) | App Note*/ Complete Kit |
| p65 | NF-κB | Set 2 (W1331) | App Note* |
| Phospho-p65 (Ser 536) | NF-κB | Set 2 (W1331) | App Note*/ Complete Kit |
| Rb (Retinoblastoma Tumor Suppressor) | Cell Cycle | Set 2 (W1331) | App Note |
| Phospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 780) | Cell Cycle | Set 2 (W1331) | App Note |
| Phospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 807/811) | Cell Cycle | Set 2 (W1331) | App Note/ Complete Kit |
| Ribosomal Protein S6 |
PI3K/mTOR/AKT | Set 1 (W1201) | App Note |
| Phospho-Ribosomal Protein S6 (Ser 240/244) |
PI3K/mTOR/AKT | Set 1 (W1201) | App Note/ Complete Kit |
| Smad1 | BMP | Set 1 (W1201) | App Note |
| Phospho-Smad1 (Ser 463/465) | BMP | Set 1 (W1201) | App Note |
| Smad2 | TGF-β | Set 2 (W1331) | App Note |
| Phospho-Smad2 (Ser 465/467) | TGF-β | Set 2 (W1331) | App Note |
| STAT1 | JAK/STAT | Set 1 (W1201) | App Note |
| Phospho-STAT1 (Tyr 701) | JAK/STAT | Set 1 (W1201) | App Note |
| Phospho-STAT1 (Ser 727) | JAK/STAT | Set 1 (W1201) | App Note |
| Phospho-STAT2 (Tyr 690) | JAK/STAT | Set 1 (W1201) | App Note |
| STAT 3 |
JAK/STAT | Set 1 (W1201) | App Note/ Complete Kit |
| Phospho-STAT 3 (Tyr 705) | JAK/STAT | Set 1 (W1201) | App Note/ Complete Kit |
Targeted Protein Degradation Series
Learn more about our solutions for targeted protein degradation.
| Human Cellular Target | Degrader(s) | Lumit® Immunoassay Cellular System (Cat.#) |
Application Note / Complete Kit |
|---|---|---|---|
| AKT |
PROTAC MS-21 | Set 1 (W1201) | App Note |
| B-cell lymphoma 6 protein (BCL-6) | BI-3802 | Set 1 (W1201) | App Note |
| BRD4 |
PROTACs ARV-771, dBET6 | Set 1 (W1201) | App Note/ Complete Kit |
| Burton's Tyrosine Kinase (BTK) |
PROTAC MT-802 | Set 1 (W1201) | App Note/ Complete Kit |
| ER (Estrogen Receptor) | Fulvestrant | Set 1 (W1201) | App Note |
| SMARCA2 (BRM) |
PROTAC ACBI-1 | See Application Note for details | App Note/ Complete Kit |
| SMARCA4 (BRG1) |
PROTAC ACBI-1 | Set 2 (W1331) | App Note/ Complete Kit |
| STAT3 |
PROTAC SD-36 | Set 1 (W1201) | App Note/ Complete Kit |
Lumit® Immunoassay Cellular System の測定原理
シンプルな１プレートプロトコル、洗浄不要 ！
Lumit® イムノアッセイ vs. ウェスタンブロッティング
総タンパク質およびリン酸化タンパク質の定量的検出
創薬プロセスにおける新規化合物スクリーニングへのLumit® Immunoassay Cellular Systemsの活用
ここでは、カーディフ大学医学部のTracey Martin博士が、運動性タンパク質N-WASPの研究においてLumit®イムノアッセイをどのように活用しているかを紹介しています。このプロジェクトでは、N-WASPの発現およびリン酸化状態への影響を評価するために、150種類の新規化合物をスクリーニングしています。それぞれの化合物について複数の濃度を異なる細胞種で試験しており、大量のサンプルが生成されます。当初チームはN-WASP発現量の測定にウェスタンブロッティングを使用していましたが、コストが高く、労力と時間がかかりすぎるという問題がありました。
この査読付き論文は『Nature Communications Biology』に掲載されており、Lumit®イムノアッセイを用いて、リン酸化の特定のノード（pIκBα、pAKT、pSTAT3）を通じた複数のシグナル伝達経路の活性化をモニタリングする方法が示されています。
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944C | 1 × 50μl |
Lumit® Detection Substrate C |
VB103A | 1 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 1 × 1.5ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102A | 1 × 30μl |
Lumit® Anti-Rabbit Ab-SmBiT |
W103A | 1 × 30μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 1 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310A | 1 × 500μl |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944D | 1 × 500μl |
Lumit® Detection Substrate C |
VB103B | 2 × 1ml |
Lumit® Immunoassay Buffer C, 10X |
VB115B | 1 × 15ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102B | 1 × 300μl |
Lumit® Anti-Rabbit Ab-SmBiT |
W103B | 1 × 300μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 2 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310B | 1 × 5ml |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944D | 10 × 500μl |
Lumit® Detection Substrate C |
VB103B | 20 × 1ml |
Lumit® Immunoassay Buffer C, 10X |
VB115B | 10 × 15ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102B | 10 × 300μl |
Lumit® Anti-Rabbit Ab-SmBiT |
W103B | 10 × 300μl |
Antibody Dilution Buffer |
W108A | 10 × 1ml |
|
GF-AFC Substrate |
G608A | 20 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310B | 10 × 5ml |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944C | 1 × 50μl |
Lumit® Detection Substrate C |
VB103A | 1 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 1 × 1.5ml |
Lumit® Anti-Rabbit Ab-LgBiT |
W104A | 1 × 30μl |
Lumit® Anti-Mouse Ab-SmBiT |
W105A | 1 × 30μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 1 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310A | 1 × 500μl |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944D | 1 × 500μl |
Lumit® Detection Substrate C |
VB103B | 2 × 1ml |
Lumit® Immunoassay Buffer C, 10X |
VB115B | 1 × 15ml |
Lumit® Anti-Rabbit Ab-LgBiT |
W104B | 1 × 300μl |
Lumit® Anti-Mouse Ab-SmBiT |
W105B | 1 × 300μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 2 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310B | 1 × 5ml |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944D | 10 × 500μl |
Lumit® Detection Substrate C |
VB103B | 20 × 1ml |
Lumit® Immunoassay Buffer C, 10X |
VB115B | 10 × 15ml |
Lumit® Anti-Rabbit Ab-LgBiT |
W104B | 10 × 300μl |
Lumit® Anti-Mouse Ab-SmBiT |
W105B | 10 × 300μl |
Antibody Dilution Buffer |
W108A | 10 × 1ml |
|
GF-AFC Substrate |
G608A | 20 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310B | 10 × 5ml |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
Digitonin |
G944C | 2 × 50μl |
Lumit® Detection Substrate C |
VB103A | 2 × 200μl |
Lumit® Immunoassay Buffer C, 10X |
VB115A | 2 × 1.5ml |
Lumit® Anti-Mouse Ab-LgBiT |
W102A | 1 × 30μl |
Lumit® Anti-Rabbit Ab-SmBiT |
W103A | 1 × 30μl |
Lumit® Anti-Rabbit Ab-LgBiT |
W104A | 1 × 30μl |
Lumit® Anti-Mouse Ab-SmBiT |
W105A | 1 × 30μl |
Antibody Dilution Buffer |
W108A | 1 × 1ml |
GF-AFC Substrate |
G608A-C | 1 × 10μl |
Lumit® Lysis Buffer II, 10X |
VB310A | 2 × 500μl |
SDS
Search for SDS分析証明書
使用制限
For Research Use Only. Not for Use in Diagnostic Procedures.保存条件
Please see the Protocol for detailed storage conditions.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
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