GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line

Part Numbers: E8520

NF-κB応答エレメントつきフェラーゼレポーター導入済み細胞株

  • NF-κB活性を変化させるあらゆる細胞内応答について迅速、簡便に分析
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選択製品のカタログ番号: E8520

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GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line
2 vials
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GloResponse™ Luciferase Reporter Cell Linesは、目的のパスウェイに対する特異的な応答エレメントを有するpGL4ベースのベクターを安定にトランスフェクションしたHEK293細胞です。これらの細胞株は、パフォーマンスの向上したpGL4レポーターベクターファミリーの特性をそのまま引き継いでいます。不安定化したluc2Pルシフェラーゼレポーターは、転写ダイナミクスに対する優れた応答性を捕らえることができます。luc2P遺伝子は哺乳動物細胞での発現を向上させるためにコドンが最適化され、pGL4プラスミドの骨格はレポーター発現のバックグラウンドを低減するための改良が施されています。これらの改良により、目的パスウェイの活性化時により高い誘導が得られるようになりました。これにより標的とするパスウェイを解析するためのレポーターアッセイシステムを迅速に開発することができます。これらのアッセイは通常、他のアッセイフォーマットよりも大きな応答ダイナミクス(誘導倍率)、高いZ'値を示すため、ハイスループットスクリーニングにも最適です。GloResponse™ Cell Linesは様々なシグナル伝達パウェイを研究するために開発され、これらのパスウェイのアクティベータはHEK293細胞に内在するネイティブ、あるいはトランスフェクションにより導入した非ネイティブのアクティベータとして研究することができます。

NF-κB-REはNF-κB転写因子複合体のDNA結合配列で、炎症制御、免疫応答、細胞増殖、アポトーシスに関与します。GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line は、NF-κB活性を変化させるあらゆる細胞内応答について迅速、簡便に分析できるようにデザインされています。
GloResponse NF-KB-RE-luc2P HEK293 cells response to TNF-alpha titration.

GloResponse™ NF-κB-RE-luc2P HEK293 cells response to TNF-α titration. A total of 10,000 GloResponse™ NF-κB-RE-luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and twofold serial dilutions of TNF-α were added to induce reporter gene expression. After 5 hours of induction in a tissue culture incubator, luciferase activity was quantified using the ONE-Glo™ Luciferase Assay System Reagent on the Berthold® LB 96 V Luminometer. n = 8 for each data point.

製品仕様

選択製品の構成品内容

Item Part # Size

NF-κB-luc2P HEK293 Cell Line

E852A 2 × 1 vial

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

XX

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

Use of Genetically Modified Microorganisms (GMM)
Information for European Customers: These products are genetically modified as described in Promega technical literature. As a condition of sale, use of this product must be in accordance with all applicable local guidelines on the contained use of genetically modified microorganisms, including the Directive 2009/41/EC of the European Parliament and of the Council.

HEK293 cells were obtained under license from AdVec Inc.

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