GPCRは広範な生物学的機能を制御しており、薬剤開発の最も重要な標的対象の1つです。また、GPCRシグナリングパスウェイは、Gタンパク質αサブユニットをもとにGs、Gi/o、Gqの3つのクラスに分類されています。GloResponse™ CRE-luc2P HEK293 Cell LineはcAMPおよびCREを通じたシグナルであるGsおよび Gi/o共役GPCRの研究やスクリーニングアッセイに使用することができます。また、カルシウムイオンおよびNFAT-REを通じたシグナルであるGq共役GPCRの場合はGloResponse™ NFAT-RE-luc2P HEK293 Cell Lineを使用します。
GloResponse™ NFAT-RE-luc2P HEK293 cells response to PMA titration. A total of 10,000 GloResponse™ NFAT-RE-luc2P HEK293 cells per well were dispensed into each well of a 384-well plate, and threefold serial dilutions of PMA were added to induce reporter gene expression. After 16 hours of induction in a tissue culture incubator, luciferase activity was quantified using the Dual-Glo® Luciferase Assay System Reagent on the Berthold® LB 96 V Luminometer. n = 8 for each data point.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
GloResponse™ NFAT-RE-luc2P HEK293 Cell Line |
E851A | 2 × 1 vial |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Use of Genetically Modified Microorganisms (GMM)
Information for European Customers: These products are genetically modified as described in Promega technical literature. As a condition of sale, use of this product must be in accordance with all applicable local guidelines on the contained use of genetically modified microorganisms, including the Directive 2009/41/EC of the European Parliament and of the Council.
HEK293 cells were obtained under license from AdVec Inc.
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