Minimal Promoter-Driven Firefly Luciferase Vectors with Hygromycin Selection

Part Numbers: E8441, E8451, E8461

E6751_pGL4-20-luc2-Puro-Vector_3
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最小限プロモーターとルシフェラーゼレポーター遺伝子の上流に応答エレメントをクローンする

  • 最小限プロモーター (minP) は応答エレメントの上流にクローンされている場合にルシフェラーゼの発現を駆動
  • 3種の異なる組換えホタルルシフェラーゼ遺伝子 (luc2luc2Pluc2CP) を提供
  • ハイグロマイシンで安定発現細胞の選抜も可能

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選択製品のカタログ番号: E8441

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Minimal Promoter-Driven Firefly Luciferase Vectors with Hygromycin Selection
luc2/20µg
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特定のシグナルパスウェイの活性化を示す細胞株を構築することは、シグナルパスウェイの構成要素を解析する上で非常に有用です。これらのツールは最小限プロモーター (minP) の上流に応答配列を挿入することにより作成されます。プロメガは、最適化されたluc2 ホタルルシフェラーゼ遺伝子を用いて様々なパスウェイの活性をレポーティングするためにデザインされたpGL4ベースのベクターを提供しています。これらのベクターにはハイグロマイシン耐性選択マーカーが組み込まれており、トランジェントなトランスフェクション実験だけでなく安定細胞株の選択も行うことができます。

pGL4パスウェイベクターの選択ガイドはこちら

全てのpGL4 ベクターはこちら

 

パスウェイレポーターの構築には、それぞれ異なる組換えホタルルシフェラーゼ遺伝子 (luc2, luc2P, luc2CP) を持つ3種類の 最小限プロモーター(minP) ベクターがご利用いただけます。Luc2遺伝子からはほとんどの変則的に働く転写因子結合サイトが除去され、コドンの最適化により哺乳動物での発現が向上しました。luc2P、luc2CP およびRapidResponse™ 遺伝子はluc2遺伝子にルシフェラーゼの細胞内半減期に影響を及ぼす分解配列を付加したものです。RapidResponse™ 遺伝子は刺激に対してより素早く応答しますが、シグナル強度を犠牲にしています。luc2P遺伝子(半減期 1時間)はluc2(半減期 3時間)よりもより早く応答しますが、中程度のシグナル強度を示し、luc2CP(半減期 0.4時間)はさらに迅速に応答しますが、シグナル強度は低く抑えられています。このminPベクターは選択マーカー(ハイグロマイシン)有/無の2タイプがご利用いただけます。研究をスピードアップするために、すでに応答配列を組み込んだpGL4.27ベースのプレデザインされたパスウェイベクターをいくつか揃えています。これらのいくつかは安定細胞株 (GloResponse™ Cell Lines) もご用意しています。


  • プレデザインベクター:クロニングする手間やアッセイによる確認が不要
  • レポーターの発現増加: 哺乳動物発現のためのコドン最適化
  • バックグラウンドとアーティファクトのリスクの低減: 変則的なDNA調節エレメントおよび転写因子結合サイトを除去
  • 応答時間を改善: ルシフェラーゼ遺伝子の不安化させてたRapid Response™ テクノロジー
  • トランジェント/安定発現でも: 哺乳動物用の選択マーカーを選択可能

  • 転写調節
  • ウイルス-細胞 相互作用
  • 化合物スクリーニング
  • 翻訳後修飾
  • GPCR シグナリング
  • 細胞内シグナリング
  • プロモーター分析

Protocols & Applications Guide もご参照ください。


【ライセンス】pGL4ベクターシリーズ

About the pGL4 Reporter Vectors

The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

5951MA-W

pGL4.26[luc2/minP/Hygro] Vector GenBank® Accession Number DQ904458 and vector sequence text file.

5952MA-W

pGL4.27[luc2P/minP/Hygro] Vector GenBank® Accession Number DQ904459 and vector sequence text file.

5953MA-W

pGL4.28[luc2CP/minP/Hygro] Vector GenBank® Accession Number DQ904460 and vector sequence text file.

製品仕様

You are viewing: E8441 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.26[luc2/minP/Hygro] Vector

E844A 1 × 20μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

製品仕様

You are viewing: E8451 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.27[luc2P/minP/Hygro] Vector

E845A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

製品仕様

You are viewing: E8461 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.28[luc2CP/minP/Hygro] Vector

E846A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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