Additional Signaling Pathway Analysis Response Element Firefly Luciferase Vectors

Part Numbers: E4111, E4611, E8471, E8481, E8491, E1340, E1350

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シグナル伝達経路にはクローニング不要のReady to Useレポーターベクター

  • 核内応答、キナーゼ、その他シグナル伝達経路に関わる数々の応答エレメント
  • 哺乳動物発現のためにコドンを最適化したホタルルシフェラーゼ遺伝子(半減期1時間)
  • ハイグロマイシン耐性選択マーカーが組み込まれており、トランジェントなトランスフェクション実験だけでなく安定細胞株の選択も可能

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選択製品のカタログ番号: E4111

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Additional Signaling Pathway Analysis Response Element Firefly Luciferase Vectors
AP1/20µg
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特定のシグナルパスウェイの活性化を示す細胞株を構築することは、シグナルパスウェイの構成要素を解析する上で非常に有用です。これらのツールは最小限プロモーター (minP) の上流に応答配列を挿入することにより作成されます。プロメガは、最適化されたluc2 ホタルルシフェラーゼ遺伝子を用いて様々なパスウェイの活性をレポーティングするためにデザインされたpGL4ベースのベクターを提供しています。これらのベクターにはハイグロマイシン耐性選択マーカーが組み込まれており、トランジェントなトランスフェクション実験だけでなく安定細胞株の選択も行うことができます。

pGL4パスウェイベクターの選択ガイドはこちら

全てのpGL4 ベクターはこちら

 

パスウェイレポーターの構築には、それぞれ異なる組換えホタルルシフェラーゼ遺伝子 (luc2, luc2P, luc2CP) を持つ3種類の 最小限プロモーター(minP) ベクターがご利用いただけます。Luc2遺伝子からはほとんどの変則的に働く転写因子結合サイトが除去され、コドンの最適化により哺乳動物での発現が向上しました。luc2P、luc2CP およびRapidResponse™ 遺伝子はluc2遺伝子にルシフェラーゼの細胞内半減期に影響を及ぼす分解配列を付加したものです。RapidResponse™ 遺伝子は刺激に対してより素早く応答しますが、シグナル強度を犠牲にしています。luc2P遺伝子(半減期 1時間)はluc2(半減期 3時間)よりもより早く応答しますが、中程度のシグナル強度を示し、luc2CP(半減期 0.4時間)はさらに迅速に応答しますが、シグナル強度は低く抑えられています。このminPベクターは選択マーカー(ハイグロマイシン)有/無の2タイプがご利用いただけます。研究をスピードアップするために、すでに応答配列を組み込んだpGL4.27ベースのプレデザインされたパスウェイベクターをいくつか揃えています。これらのいくつかは安定細胞株 (GloResponse™ Cell Lines) もご用意しています。


  • プレデザインベクター:クロニングする手間やアッセイによる確認が不要
  • レポーターの発現増加: 哺乳動物発現のためのコドン最適化
  • バックグラウンドとアーティファクトのリスクの低減: 変則的なDNA調節エレメントおよび転写因子結合サイトを除去
  • 応答時間を改善: ルシフェラーゼ遺伝子の不安化させてたRapid Response™ テクノロジー
  • トランジェント/安定発現でも: 哺乳動物用の選択マーカーを選択可能

  • 転写調節
  • ウイルス-細胞 相互作用
  • 化合物スクリーニング
  • 翻訳後修飾
  • GPCR シグナリング
  • 細胞内シグナリング
  • プロモーター分析

Protocols & Applications Guide もご参照ください。


【ライセンス】pGL4ベクターシリーズ

Sequence Information

pGL4.29[luc2P/CRE/Hygro] Vector Accession Number DQ904461 and vector sequence text file.

pGL4.30[luc2P/NFAT-RE/Hygro] Vector Accession Number DQ904462 and vector sequence text file.

pGL4.32[luc2P/NF-κB-RE/Hygro] Vector Accession Number EU581860 and vector sequence text file.

pGL4.33[luc2P/SRE/Hygro] Vector Accession Number FJ773212 and vector sequence text file.

pGL4.34[luc2P/SRF-RE/Hygro] Vector Accession Number FJ773213 and vector sequence text file.

pGL4.44[luc2P/AP1 RE/Hygro] Vector Accession Number JQ858516 and vector sequence text file.

pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector Accession Number JX099537 and vector sequence text file.

Activator/Pathway Transcription Factor Binding Site Vector Name Example Data
cAMP/PKA
CREB
Cyclic AMP Response Element
pGL4.29[luc2P/CRE/Hygro]
6045MC_PanelA_450px
High levels of reporter induction for cAMP Response Element containing vector in transient transfections. pGL4.29 Vector was transiently transfected into HEK293 cells. After 24 hours, reporter gene expression was induced by adding 100uM forskolin for 5 hours. Noninduced controls were treated with an equivalent amount of DMSO. Luciferase activity was measured using the Bright-Glo™ Luciferase Assay System (Cat.# E2610). Results will vary depending on the cell line and the transfection protocol used.
NF-κB
NF-κB
Nuclear Factor κB Response Element
pGL4.32[luc2P/NF-κB-RE/Hygro]
6045MC_PanelC_450px
High levels of reporter induction for Nuclear Factor κB (NF- κB) Response Element containing vector in transient transfections. pGL4.32 Vector was transiently transfected into HEK293 cells. After 24 hours, reporter gene expression was induced by 20ng/ml of TNFα for 5 hours. Noninduced controls were treated with an equivalent amount of standard media. Luciferase activity was measured using the ONE-Glo™ Luciferase Assay System (Cat.# E6110). Results will vary depending on the cell line and the transfection protocol used.
Calcium/Calcineurin
NFAT
Nuclear Factor of Activated T-Cells (NFAT) Response Element
pGL4.30[luc2P/NFAT-RE/Hygro]
6045MC_PanelB_450px

High levels of reporter induction for Nuclear Factor of Activated T-cells (NFAT) Response Element containing vector in transient transfections. pGL4.30 Vector was transiently transfected into HEK293 cells. After 24 hours, reporter gene expression was induced by adding 1μM ionomycin plus 10ng/ml PMA for 17 hours. Noninduced controls were treated with an equivalent amount of DMSO. Luciferase activity was measured using the Bright-Glo™ Luciferase Assay System (Cat.# E2610). Results will vary depending on the cell line and the transfection protocol used.

MAP/ERK
Elk-1/SRF
Serum Response Element
pGL4.33[luc2P/SRE/Hygro]
6045MC_PanelD_450px
High levels of reporter induction for Serum Response Element (SRE) containing vector in transient transfections. pGL4.33 Vector was transiently transfected into HEK293 cells. Four hours after transfection, cells were changed into serum-starved media. After 24 hours following transfection, reporter gene expression was induced 20% FBS plus 10ng/ml PMA for 6 hours. Noninduced controls were treated with an equivalent amount of standard media. Luciferase activity was measured using the ONE-Glo™ Luciferase Assay System (Cat.# E6110). Results will vary depending on the cell line and the transfection protocol used.
RhoA
SRF
Serum Response Factor Response Element
pGL4.34[luc2P/SRF-RE/Hygro]
6045MC_PanelE_450px
High levels of reporter induction for Serum Response Factor Response Element (SRF-RE) containing vector in transient transfections. pGL4.34 Vector was transiently transfected into HEK293 cells. Four hours after transfection, cells were changed into serum-starved media. After 24 hours following transfection, reporter gene expression was induced 20% FBS for 6 hours. Noninduced controls were treated with an equivalent amount of standard media. Luciferase activity was measured using the ONE-Glo™ Luciferase Assay System (Cat.# E6110). Results will vary depending on the cell line and the transfection protocol used.
MAPK/JNK
AP1
AP1 Response Element (AP1 RE)
pGL4.44 [luc2P/AP1 RE/Hygro]
10719MA_425px
Representative data for pGL4.44[luc2P/AP1 RE/Hygro] Vector in HEK293 cells upon stimulation with PMA. HEK293 cells were transiently transfected with pGL4.44[luc2P/AP1 RE/Hygro] and pGL4.75[hRluc/CMV] Vectors and assayed in a 96-well format as indicated in the protocol after six hours stimulation with PMA. Firefly luciferase luminescence normalized to the Renilla luciferase control is shown, with error bars indicating the S.E.M. for five replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time.
Wnt
TCF-LEF
TCF-LEF Response Element (TCF-LEF RE)
pGL4.49[luc2P/TCF-LEF RE/Hygro]
10812MA_465px
Representative data for pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector in HEK293 cells upon stimulation with mWnt3a or LiCl. HEK293 cells were transiently transfected with pGL4.49[luc2P/TCF-LEF RE/Hygro] and assayed in 96-well format after 8 hours stimulation with mWnt3a or LiCl as indicated in the protocol. Firefly luciferase luminescence normalized to untreated cells is shown, with error bars indicating S.E.M. for three replicates. Luminescence was detected after addition of ONE-Glo™ Reagent, using a GloMax® 96 instrument with a 0.5 second integration time.
5257ML2

製品仕様

You are viewing: E4111 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration
pGL4.44[luc2P/AP1 RE/Hygro] Vector E411A 1 × 20μg 1μg/μl

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

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パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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You are viewing: E4611 Change Configuration

選択製品の構成品内容

Item Part # Size Concentration
pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector E461A 1 × 20μg 1μg/μl

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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You are viewing: E8471 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.29[luc2P/CRE/Hygro] Vector

E847A 1 × 20μg

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

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パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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You are viewing: E8481 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.30[luc2P/NFAT-RE/Hygro] Vector

E848A 1 × 20μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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You are viewing: E8491 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.32[luc2P/NF-κB-RE/Hygro] Vector

E849A 1 × 20μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

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パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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You are viewing: E1340 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.33[luc2P/SRE/Hygro] Vector

E134A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

製品仕様

You are viewing: E1350 Change Configuration

選択製品の構成品内容

Item Part # Size

pGL4.34[luc2P/SRF-RE/Hygro] Vector

E135A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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