Additional Signaling Pathway Analysis Response Element Firefly Luciferase Vectors
Reporter Vectors Ready to Use in Your Signaling Pathway Without Cloning
- Offers a number of response elements that are involved in nuclear response, kinase and other signaling pathways
- Each vector includes an optimized firefly luciferase gene that codes a reporter with a one-hour half-life
- Hygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line
Catalog Number:
Choose a response element
Size
Catalog Number: E4111
Catalog Number: E4611
Catalog Number: E8471
Catalog Number: E8481
Catalog Number: E8491
Catalog Number: E1340
Catalog Number: E1350
パスウェイレポーターの構築には、それぞれ異なる組換えホタルルシフェラーゼ遺伝子 (luc2, luc2P, luc2CP) を持つ３種類の 最小限プロモーター(minP) ベクターがご利用いただけます。Luc2遺伝子からはほとんどの変則的に働く転写因子結合サイトが除去され、コドンの最適化により哺乳動物での発現が向上しました。luc2P、luc2CP およびRapidResponse™ 遺伝子はluc2遺伝子にルシフェラーゼの細胞内半減期に影響を及ぼす分解配列を付加したものです。RapidResponse™ 遺伝子は刺激に対してより素早く応答しますが、シグナル強度を犠牲にしています。luc2P遺伝子（半減期 1時間）はluc2（半減期 3時間）よりもより早く応答しますが、中程度のシグナル強度を示し、luc2CP（半減期 0.4時間）はさらに迅速に応答しますが、シグナル強度は低く抑えられています。このminPベクターは選択マーカー（ハイグロマイシン）有/無の2タイプがご利用いただけます。研究をスピードアップするために、すでに応答配列を組み込んだpGL4.27ベースのプレデザインされたパスウェイベクターをいくつか揃えています。これらのいくつかは安定細胞株 (GloResponse™ Cell Lines) もご用意しています。
- プレデザインベクター：クロニングする手間やアッセイによる確認が不要
- レポーターの発現増加: 哺乳動物発現のためのコドン最適化
- バックグラウンドとアーティファクトのリスクの低減: 変則的なDNA調節エレメントおよび転写因子結合サイトを除去
- 応答時間を改善: ルシフェラーゼ遺伝子の不安化させてたRapid Response™ テクノロジー
- トランジェント/安定発現でも: 哺乳動物用の選択マーカーを選択可能
- 転写調節
- ウイルス-細胞 相互作用
- 化合物スクリーニング
- 翻訳後修飾
- GPCR シグナリング
- 細胞内シグナリング
- プロモーター分析
Protocols & Applications Guide もご参照ください。
【ライセンス】pGL4ベクターシリーズ
Sequence Information
pGL4.29[luc2P/CRE/Hygro] Vector Accession Number DQ904461 and vector sequence text file.
pGL4.30[luc2P/NFAT-RE/Hygro] Vector Accession Number DQ904462 and vector sequence text file.
pGL4.32[luc2P/NF-κB-RE/Hygro] Vector Accession Number EU581860 and vector sequence text file.
pGL4.33[luc2P/SRE/Hygro] Vector Accession Number FJ773212 and vector sequence text file.
pGL4.34[luc2P/SRF-RE/Hygro] Vector Accession Number FJ773213 and vector sequence text file.
pGL4.44[luc2P/AP1 RE/Hygro] Vector Accession Number JQ858516 and vector sequence text file.
pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector Accession Number JX099537 and vector sequence text file.
| Activator/Pathway | Transcription Factor | Binding Site | Vector Name | Example Data |
|---|---|---|---|---|
|
cAMP/PKA
|
CREB
|
Cyclic AMP Response Element
|
pGL4.29[luc2P/CRE/Hygro]
|
|
|
NF-κB
|
NF-κB
|
Nuclear Factor κB Response Element
|
pGL4.32[luc2P/NF-κB-RE/Hygro]
|
|
|
Calcium/Calcineurin
|
NFAT
|
Nuclear Factor of Activated T-Cells (NFAT) Response Element
|
pGL4.30[luc2P/NFAT-RE/Hygro]
|
High levels of reporter induction for Nuclear Factor of Activated T-cells (NFAT) Response Element containing vector in transient transfections. pGL4.30 Vector was transiently transfected into HEK293 cells. After 24 hours, reporter gene expression was induced by adding 1μM ionomycin plus 10ng/ml PMA for 17 hours. Noninduced controls were treated with an equivalent amount of DMSO. Luciferase activity was measured using the Bright-Glo™ Luciferase Assay System (Cat.# E2610). Results will vary depending on the cell line and the transfection protocol used. |
|
MAP/ERK
|
Elk-1/SRF
|
Serum Response Element
|
pGL4.33[luc2P/SRE/Hygro]
|
|
|
RhoA
|
SRF
|
Serum Response Factor Response Element
|
pGL4.34[luc2P/SRF-RE/Hygro]
|
|
|
MAPK/JNK
|
AP1
|
AP1 Response Element (AP1 RE)
|
pGL4.44
[luc2P/AP1 RE/Hygro]
|
|
|
Wnt
|
TCF-LEF
|
TCF-LEF Response Element (TCF-LEF RE)
|
pGL4.49[luc2P/TCF-LEF RE/Hygro]
|
|
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.44[luc2P/AP1 RE/Hygro] Vector | E411A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size | Concentration |
|---|---|---|---|
| pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector | E461A | 1 × 20μg | 1μg/μl |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.29[luc2P/CRE/Hygro] Vector |
E847A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.30[luc2P/NFAT-RE/Hygro] Vector |
E848A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.32[luc2P/NF-κB-RE/Hygro] Vector |
E849A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.33[luc2P/SRE/Hygro] Vector |
E134A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.34[luc2P/SRF-RE/Hygro] Vector |
E135A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,728,118.
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ストレス応答シグナル経路解析用（最小限プロモーター駆動）ホタルルシフェラーゼベクター
細胞のストレス応答シグナル経路における様々な応答エレメントを含むベクター（ルシフェラーゼレポーターとハイグロマイシン耐性遺伝子を含む）
E3641, E3651, E3661, E4131, E3751, E4001, E4121
サイトカインシグナル伝達経路解析用(最小限プロモーターを含む) ホタルルシフェラーゼベクター
pGL4.45[luc2P/ISRE/Hygro]、pGL4.47[luc2P/SIE/Hygro]、 pGL4.48[luc2P/SBE/Hygro] 、pGL4.52[luc2P/STAT5RE/Hygro] ベクターは様々なサイトカインシグナル経路の研究に使用されます
E4041, E3671, E4651, E4141
最小限プロモーターが駆動するホタルルシフェラーゼベクター
pGL4.23[luc2/minP]、pGL4.24[luc2P/minP] 、pGL4.25[luc2CP/minP] ベクターは、最小限プロモーター とホタルルシフェラーゼ遺伝子の上流に応答配列をクローニングするためのベクターです。
E8411, E8421, E8431
最小限プロモーターが駆動するホタルルシフェラーゼベクター（ハイグロマイシン耐性）
pGL4.26[luc2/minP/Hygro]、pGL4.27[luc2P/minP/Hygro]、 pGL4.28[luc2CP/minP/Hygro] ベクターは、最小限プロモーター とホタルルシフェラーゼ遺伝子（安定性有り/なし）の上流に応答エレメントをクローニングするためのベクターです。
E8441, E8451, E8461
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ONE-Glo™ Luciferase Assay System
ハイスループット、ウルトラハイスループットアプリケーションにパフォーマンスを向上させた、頑健なホタルルシフェラーゼアッセイ
E6110, E6120, E6130
Bright-Glo™ Luciferase Assay System
ロボットを用いた連続処理にも使える高感度レポーターアッセイ
E2610, E2620, E2650
Steady-Glo® Luciferase Assay System
マルチウェルプレートでのバッチ処理用、長時間発光の高感度レポーターアッセイ
E2510, E2520, E2550
ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay
単一ウェルでレポーター活性と細胞生存性マーカーの高感度検出
E7110, E7120
