E1310_pGL4-50-luc2-3aCMV-3aHygro--Vector-20ug_3 new-kit-box

Vectors That Are Highly Expressed in Mammalian Cell Lines for Animal Imaging Studies

  • Two selectable marker options for creating stable mammalian cell lines
  • Luciferase-expressing cells are used in animal models to study in vivo changes in cell physiology
  • Firefly luciferase expression is driven by CMV promoter

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Catalog Number: E1310

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Catalog Number: E1320

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すべてのpGL4 Vectorを見る

pGL4 Luciferase Reporter Vectorsは哺乳類細胞での発現に最適化された次世代のレポーター遺伝子ベクターです。様々な構成のpGL4 Vectorsがあります。pGL4.50とpGL4.51 Vectorはホタルルシフェラーゼの合成遺伝子であるluc2遺伝子が強力な構成的プロモーターであるCMV(cytomegalovirus)の下流に配置したベクターです。これらのベクターは試験を行った様々な細胞株で高レベルな発現が認められました。ハイグロマイシンやネオマイシンといった選択マーカーを追加することで安定発現株の作成が可能になります。恒常的にルシフェラーゼを発現する細胞株は動物モデルに使用可能であり、細胞の生理機能の変動をin vivoで研究することができます。


  • あらかじめ作製されたルシフェラーゼ発現ベクター
  • Luc2ルシフェラーゼは最も高発現
  • 安定発現株作製用薬剤選択マーカー

【ライセンス】pGL4

【Q&A】pGL4

7723MA-W

pGL4.50[luc2/CMV/Hygro] Vector GenBank® Accession Number EU921840 and vector sequence text file.

7724MA-W

pGL4.51[luc2/CMV/Neo] Vector GenBank® Accession Number  EU921841 and vector sequence text file.

Specifications

Catalog Number:

選択製品の構成品内容

Item Part # Size

pGL4.50[luc2/CMV/Hygro] Vector

E131A 1 × 20μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

選択製品の構成品内容

Item Part # Size

pGL4.51[luc2/CMV/Neo] Vector

E132A 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 7,728,118.

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