pmirGLO Dual-Luciferase miRNA Target Expression Vector

Part Numbers: E1330

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ホタルルシフェラーゼ遺伝子の3’末端側にmiRNA標的サイトを導入し、マイクロRNA(miRNA)活性を評価

  • レポーター活性はmiRNA活性に相関
  • 最適化した luc2 レポーターは最大の発現パフォーマンス、Renilla ルシフェラーゼ遺伝子で補正が可能
  • 中程度の発現強度を示すヒト-ホスホグリセレートキナーゼ(PGK)プロモーターは、強力なプロモーターでは難しい高感度な分析が可能

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選択製品のカタログ番号: E1330

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pmirGLO Dual-Luciferase miRNA Target Expression Vector
20μg
Your price:
pmirGLO Dual-Luciferase miRNA Target Expression Vectorは、ホタルルシフェラーゼ遺伝子(luc2)の3’末端側にmiRNA標的サイトを導入し、マイクロRNA(miRNA)活性を定量的に評価するためにデザインされています。ホタルルシフェラーゼは第1のレポーター遺伝子として機能し、ホタルルシフェラーゼ発現の低下は、クローニングしたmiRNA標的配列に対して内在性または細胞内に導入したmiRNAが結合したことを示します。本ベクターはプロメガのDual-Luciferaseテクノロジーがベースになっており、mRNAの調節をモニタリン グする第1レポーターであるホタルルシフェラーゼ(luc2)と標準化や選択用のコントロールレポーターとして機能するウミシイタケルシフェラーゼ(hRluc-neo)が含まれています。

  • miRNAまたはmiRNA結合サイトの機能測定
  • luc2は最大の発現パフォーマンス
  • ウミシイタケ-ネオマイシン耐性カセット(hRluc-neo)は、遺伝子発現の補正用コントロールレポーターおよび安定細胞株の選択用に利用できます。
  • 中程度の発現強度を示すヒト-ホスホグリセレートキナーゼ(PGK)プロモーターは非ウイルス性のユニバーサルプロモーターで広範な細胞株で機能(酵母、ラット、マウス、ヒト)し、強力なプロモーターでは難しい高感度な分析が可能

  • miRNA 検出
  • miRNA 結合領域の分析
  • 転写物の安定性研究
  • 3´-UTR 分析

【ライセンス】pmirGLO
Normalized luciferase activity using the pmirGLO Vector with an mi-R21 target sequence.

Normalized luciferase activity using the pmirGLO Vector with an mi-R21 target sequence.

Sequence Information

pmirGLO Vector GenBank® Accession Number FJ376737 and vector sequence text file.

Mechanism of action of the pmirGLO Vector.

Mechanism of action of the pmirGLO Vector.

製品仕様

選択製品の構成品内容

Item Part # Size

Oligo Annealing Buffer

C838A 1 × 1ml

pmirGLO Vector

E133A 1 × 20μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

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パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

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