Promoterless Firefly Luciferase Basic Vectors
Reporter Vectors that Lack a Promoter and Offer Different Reporter Protein Stabilities
- Designed for cloning a putative promoter element for investigation of gene transcription control regions
- Available with three varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP
Catalog Number:
Choose a luciferase gene
Size
Catalog Number: E6651
Catalog Number: E6661
Catalog Number: E6671
The pGL4.10[luc2], pGL4.11[luc2P] and pGL4.12[luc2CP] Vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. These promoterless vectors are available with three varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP. The inserts cloned into these vectors can easily be transferred using the multiple cloning site and a unique SfiI transfer scheme.
Applications
- Transcription regulation.
- Virus-cell interactions.
- Compound screening.
- Post-translational modifications.
- Promoter analysis.
How the Engineered Firefly Luciferase Genes Work
The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and luc2CP and RapidResponse™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of firefly luciferase. The RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity, and the luc2CP (0.4-hour half-life) responds more quickly with the lowest signal intensity.
About the pGL4 Reporter Vectors
The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.
pGL4.10[luc2] Vector GenBank® Accession Number AY738222 and vector sequence text file.
pGL4.11[luc2P] Vector GenBank® Accession Number AY738223 and vector sequence text file.
pGL4.12[luc2CP] Vector GenBank® Accession Number AY738224 and vector sequence text file
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.10[luc2] Vector |
E665A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.11[luc2P] Vector |
E666A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
pGL4.12[luc2CP] Vector |
E667A | 1 × 20μg |
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
No related resources available
Related Products
類似製品
Promoterless Firefly Luciferase Vectors with Puromycin Selection
The pGL4.20[luc2/Puro]、pGL4.21[luc2P/Puro]、pGL4.22[luc2CP/Puro] ベクターはそれぞれ安定性の異なるルシフェラーゼタンパク質をコードしています。ピューロマイシンで安定発現株の選抜が可能。
E6751, E6761, E6771
Promoterless Firefly Luciferase Vectors with Hygromycin Selection
The pGL4.14[luc2/Hygro]、pGL4.15[luc2P/Hygro]、pGL4.16[luc2CP/Hygro]ベクターはそれぞれ安定性の異なるルシフェラーゼタンパク質をコードし、遺伝子発現転写調節領域の研究に使用できます。ハイグロマイシンで安定発現株の選抜が可能。
E6691, E6701, E6711
Promoterless Firefly Luciferase Vectors with Neomycin Selection
The The pGL4.17[luc2/Neo] 、pGL4.18[luc2P/Neo]、pGL4.19[luc2CP/Neo] ベクターはそれぞれ安定性の異なるルシフェラーゼタンパク質をコードし、遺伝子発現転写調節領域の研究に使用できます。ネオマイシンで安定発現株の選抜が可能。
E6721, E6731, E6741
Promoterless NanoLuc® Reporter Basic Vectors
pNL1.1[Nluc]、pNL1.2[NlucP] ベクターは推定されるプロモーター領域をクローニングして非常に明るいNanoLuc®ルシフェラーゼを発現させるために使用します。
N1001, N1011
関連製品
Steady-Glo® Luciferase Assay System
マルチウェルプレートでのバッチ処理用、長時間発光の高感度レポーターアッセイ
E2510, E2520, E2550
Bright-Glo™ Luciferase Assay System
ロボットを用いた連続処理にも使える高感度レポーターアッセイ
E2610, E2620, E2650
ONE-Glo™ Luciferase Assay System
ハイスループット、ウルトラハイスループットアプリケーションにパフォーマンスを向上させた、頑健なホタルルシフェラーゼアッセイ
E6110, E6120, E6130
Dual-Luciferase® Reporter Assay System
単一サンプルでのホタル、ウミシイタケルシフェラーゼ活性の連続測定。インジェクター2本必要。
E1910, E1960, E1980
