Nano-Glo® Dual-Luciferase® Reporter Assay System

Part Numbers: N1610, N1620, N1630, N1650, N1521, N1531, N1541, N1551

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同一サンプルよりホタルおよびNanoLuc® ルシフェラーゼ活性を超高感度に測定

  • 低発現レベルでもより高感度に測定
  • 実験レポーターとしてNanoLuc® またはホタルのどちらでも選択可能
  • 安定性の向上した試薬により柔軟にアッセイをデザイン
  • コントロールベクターと試薬10mlのセットがお買い得!(以下のプルダウンリストよりお選びください)

Size

Add a control vector (with 10ml size only)

選択製品のカタログ番号: N1610

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Nano-Glo® Dual-Luciferase® Reporter Assay System
10ml
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最新のレポーターテクノロジーは最高の感度と無限の可能性をもたらします!

Nano-Glo® Dual-Luciferase® Reporter (NanoDLR) Assay は1つのサンプルよりホタルと NanoLuc® ルシフェラーゼ 両方の活性を検出するためのアッセイです。改良されたホタルルシフェラーゼ発光反応と NanoLuc® ルシフェラーゼの明るさと感度により、従来のデュアルアッセイよりもデータの質も向上し、操作も簡便で柔軟にアッセイをデザインすることができます。

NanoDLR Assay のプロトコールでは始めにONE-Glo™ EX Luciferase Assay Reagent を使用してホタルルシフェラーゼ活性を測定します。続いて NanoDLR™ Stop & Glo® Reagent を添加することによりホタルのシグナルが消され、 NanoLuc® ルシフェラーゼ 活性の測定に必要な基質が供給されます。 

Dual-Luciferase Reporter Assay featuring NanoLuc and firefly luciferases

明るく、安定なシグナル

デュアルルシフェラーゼが同じプロモーターから発現されると、NanoDLR™ アッセイは Fluc と Nluc の両方のレポーターに対して安定した ”グロー型” の明るいシグナルを提供します。シグナルの半減期は両方のレポーターで2時間以上です。
NanoLuc and firefly dual luciferases offer brighter, more stable signal
TK-Rluc(ウミシイタケ):TK-Fluc(ホタル):キャリア DNA または TK-Nluc(NanoLuc®):TK-Fluc:キャリアDNAを 1:1:8 で HEK293細胞にDNA をトランスフェクションし、NanoDLR™, DLR™ または Dual-Glo® Dual-Luciferase Assay Systemで得られた発光シグナルをグラフのように比較しました。

より優れた感度と消光性能

ホタルルシフェラーゼ (Fluc) を強力に阻害することにより、優れたシグナル分離が実現されるため、Fluc活性が高い場合でも、少量のNanoLuc®ルシフェラーゼ (Nluc) を感度良く測定することができます。
State-of-the-art dual-luciferase assay with better quenching for greater sensitivity
This data shows the efficient quenching of the firefly luciferase signal in the NanoDLR™ Assay. Even extremely high Fluc signals are reduced to near background levels by NanoDLR™ Stop & Glo® Reagent, providing excellent signal separation and low variability without sacrificing the sensitivity of two bright luminescent readouts.

ホタルまたは NanoLuc® を実験レポーターとして、
あるいは両方を実験レポーターとして使うのもあり!

Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assayは、アッセイデザインに合わせて様々な組み合わせでご使用ください。
NanoLuc as Primary Reporter
Firefly as Primary Reporter
Two Primary Reporters
Coincidence Reporter System

NanoLuc® を実験レポーターとして

最も明るく、最も応答性の高いフォーマット

  • 細胞数が少ない場合、プレートをスケールアップする場合あるいはトランスフェクション効率が低くレポーター実験が困難な細胞の場合など。
  • 組合わせ:  NlucP =実験用、Fluc=内部標準用

ハイライトデータは、NFkBNanoLuc® PESTベクターとTK-Flucコントロールを10:1の割合でトランスフェクトしたHEK293細胞の発光値です。細胞をTNFαで処理し、処理後4時間後にNanoDLR™アッセイを使用してレポーター活性を測定しました。

Dual-luciferase assay featuring NanoLuc reporter and firefly control

ホタルを実験レポーターとして

]既存の実験用ホタルルシフェラーゼレポーターベクターをそのまま使用し、NanoLuc® を安定な内部標準用として使用

  • 標準的なレポーターアッセイフォーマット。NanoLuc®はウミシイタケルシフェラーゼの最大 1000倍明るいので、より少量のコントロール DNAで使用可能
  • 組合わせ:  FlucP = 実験用、Nluc  = 内部標準用

ハイライトデータは、NFkB応答エレメントを含むFluc-PESTベクターとTK-Nanoluc®コントロールベクターを10:1の割合でトランスフェクトしたHEK293細胞の発光値です。細胞をTNFαで処理し、処理後4時間後にNanoDLR™アッセイを使用してレポーター活性を測定しました。

Dual-luciferase assay with firefly reporter and NanoLuc control

両方を実験レポーターとして

異なる2つの生物学的応答を同時に測定

  • 情報を最大限取得するために1サンプルより2つのパスウェイを測定(例:タンパク質安定性アッセイとレポーターアッセイのマルチアッセイ)
  • 組合わせ:  NlucP =実験レポーター用または実験タンパク質安定性アッセイ用、FlucP= 実験用)

この例では、NanoDLR™アッセイを使用して、同じサンプルでタンパク質動態と遺伝子活性化を測定しました。HEK293細胞にpNLF1-HIF1A[CMV/neo] 融合コンストラクトを一過性にトランスフェクトし、低酸素応エレメントベクターである pGL4.42[luc2P/HRE/Hygro] に1:1000希釈したものを導入しました。18時間後、細胞を様々な用量の1,10-フェナントロリンで刺激し、Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assayを用いてホタルルシフェラーゼ転写レポーターおよび HIF1a-NanoLuc®融合タンパク質の発現を表示の時点でアッセイしました。

Dual-luciferase assay with both NanoLuc and firefly as reporters

コインシデンスレポーター

1つのオープンリーディングフレームから2種類のレポーターを発現

  •  HTSにおいて標的遺伝子の発現に影響する真の化合物と偽陽性ヒット化合物を区別可能
  • 構造:  Firefly-P2A-NanoLuc®-PEST construct (pNLCoI Vectors)
Coincidence dual-luciferase reporter system with NanoLuc and firefly luciferases

アッセイ試薬の安定性が向上し、繰り返しの使用が便利になり、無駄もなくなります!

NanoDLR™ アッセイの中の調製後の ONE-Glo™ EX 試薬は、室温および7℃での安定性が向上しており、予め使用量ごとに分注したり
試薬を凍結する手間がなくなりました。

右のデータは、NanoDLR™試薬の調製後の安定性が向上していることを示しています。調製後の試薬は、22℃または7℃で保存され、決められた時間に-70℃で凍結されました。解凍後、試薬を精製ホタルまたはNanoLuc®ルシフェラーゼと組み合わせ、酵素添加3分後に測定した各サンプルの発光シグナル強度を、22℃インキュベーションなしで-70℃に置いたサンプルのシグナル強度に対する相対活性として算出しました (n=3)。

Dual-luciferase assay reagents are stable for NanoLuc and firefly detection
GloMax Discover System Multimode Plate Reader

どんなワークフローあるいはスループットにも対応するマルチプレートリーダー

Nano-Glo® Dual-Luciferase® Reporter Assay の向上したシグナル安定性は、簡単 な”添加ー混和ー測定” だけのアッセイプロトコルを可能にします。試薬は、インジェクションベースのプロトコルや、Passive Lysis Buffer を用いて作成した細胞溶解液を用いて使用することができます。

ホモジニアスアッセイフォーマット: 成長培地中の細胞を直接アッセイします。両レポーターのシグナル半減期は2時間で、複数のプレートへの試薬の一括添加をサポートします。

インジェクションベース アッセイフォーマット: デュアルインジェクターを備えたGloMax® Discoverやバルク試薬ディスペンサー付きのルミノメーターを使用して、試薬を細胞に供給します。

Bioluminescence Resource Center

Learn more about how luminescence works, what makes it different from fluorescence and how you can use luminescence to fuel your own research.

製品仕様

You are viewing: N1610 Change Configuration

選択製品の構成品内容

Item Part # Size

ONE-Glo™ EX Luciferase Assay Buffer

E632A 1 × 10ml

ONE-Glo™ EX Luciferase Assay Substrate

E633A 1 × 1 vial

NanoDLR™ Stop & Glo® Buffer

N142A 1 × 10ml

NanoDLR™ Stop & Glo® Substrate

N143A 1 × 100μl

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1620 Change Configuration

選択製品の構成品内容

Item Part # Size

ONE-Glo™ EX Luciferase Assay Buffer

E632B 1 × 100ml

ONE-Glo™ EX Luciferase Assay Substrate

E633B 1 × 1 vial

NanoDLR™ Stop & Glo® Buffer

N142B 1 × 100ml

NanoDLR™ Stop & Glo® Substrate

N143B 1 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1630 Change Configuration

選択製品の構成品内容

Item Part # Size

ONE-Glo™ EX Luciferase Assay Buffer

E632A 10 × 10ml

ONE-Glo™ EX Luciferase Assay Substrate

E633A 10 × 1 vial

NanoDLR™ Stop & Glo® Buffer

N142A 10 × 10ml

NanoDLR™ Stop & Glo® Substrate

N143A 10 × 100μl

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1650 Change Configuration

選択製品の構成品内容

Item Part # Size

ONE-Glo™ EX Luciferase Assay Buffer

E632B 10 × 100ml

ONE-Glo™ EX Luciferase Assay Substrate

E633B 10 × 1 vial

NanoDLR™ Stop & Glo® Buffer

N142B 10 × 100ml

NanoDLR™ Stop & Glo® Substrate

N143B 10 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

製品仕様

You are viewing: N1521 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Dual-Luciferase® Reporter Assay System

N1610 1 × 10ml

pNL1.1.TK[Nluc/TK] Vector

N1501 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

Patent Pending.

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents.

Certain applications of this product may require licenses from others.

製品仕様

You are viewing: N1531 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Dual-Luciferase® Reporter Assay System

N1610 1 × 10ml

pNL1.1.PGK[Nluc/PGK] Vector

N1441 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.

In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.

For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.

Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents.

Patent Pending.

Certain applications of this product may require licenses from others.

製品仕様

You are viewing: N1541 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Dual-Luciferase® Reporter Assay System

N1610 1 × 10ml

pGL4.54[luc2/TK] Vector

E5061 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents.

Patent Pending.

Certain applications of this product may require licenses from others.

製品仕様

You are viewing: N1551 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Dual-Luciferase® Reporter Assay System

N1610 1 × 10ml

pGL4.53[luc2/PGK] Vector

E5011 1 × 20μg

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents.

Patent Pending.

Certain applications of this product may require licenses from others.

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