Improve live-cell assays with ViaScript™ LgBiT mRNA Delivery System

Nano-Glo® Endurazine™ and Vivazine™ Live Cell Substrates

Part Numbers: N2570, N2571, N2572, N2580, N2581, N2582, N2590

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NanoLuc® または NanoBiT® レポーター活性を数時間から数日にわたり生細胞検出

  • シグナルの安定性が飛躍的に向上し、レポーター活性のリアルタイム-カイネティック分析が可能に
  • 同じサンプルを用いた簡便な長時間 応答性タイムコース分析に
  • 内在レベルのタンパク質測定が可能な高感度(過剰発現不要)

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選択製品のカタログ番号: N2570

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Nano-Glo® Endurazine™ and Vivazine™ Live Cell Substrates
Nano-Glo® Endurazine™ Substrate/0.1ml
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Nano-Glo® 生細胞検出の概要

Nano-Glo® Live Cell Assay System による生細胞(非溶解)アッセイはシグナルの減衰により最大2時間までが限界でした。Nano-Glo® Endurazine™ および Vivazine™ Live Cell Substrate は NanoLuc® および NanoBiT® ルシフェラーゼの新たな生細胞検出用基質として数時間から数日にわたる非溶解アッセイを可能にします。この2つの基質は、細胞内エステラーゼにより触媒されるエステル加水分解の速度を低減させることで実験期間を通じて安定にフリマジンを生成します。このフリマジンは NanoLuc® および NanoBiT® ルシフェラーゼ の基質として発光反応に利用されます。

Vivazine™ substrate は 通常 Endurazine™ substrate に比べて 発光レベルが高く、シグナルの減衰速度が速いという特性を有しています。一方、Endurazine™ substrate は最大のシグナル安定性を示しますが、その他のNano-Glo® Live Cell Substrateなどに比べてシグナル強度は低く抑えられています。シグナル強度と安定性についてご自身の実験に合ったものをお選びいただくためにトライアルパックを用意しています [ Nano-Glo® Extended Live Cell Substrate Trial Pack (カタログ番号 N2590)]。

Choose the Best Combination of Signal Intensity and Stability

Comparison of Nano-Glo Live Cell Assay with Endurazine and Vivazine substrates.
Comparing Nano-Glo® Endurazine™ and Vivazine® Substrates with Nano-Glo® Live Cell Assay System. A plasmid encoding NanoLuc® luciferase expressed from the PGK promoter was transiently transfected into HEK293 cells and luminescence signal intensity was measured as indicated.

Assay Reporter Activity for Days

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Comparing Nano-Glo® Endurazine™ and Vivazine® signals over 72 hours. A plasmid encoding NanoLuc® luciferase expressed from the HSV-TK promoter was transiently transfected into HEK293 cells and luminescent signal intensity measured for 72 hours.

内在的に発現するHiBiT タグ付加タンパク質のリアルタイム測定


細胞内で LgBiTを発現する細胞内でHiBiTタグ付加タンパク質のリアルタイムな発現レベルモニタリングに使用することができます(細胞内でHiBiTはLgBiTに結合してNanoBiT® ルシフェラーゼを形成)。HiBiTタグは CRISPR-Cas9 ゲノム編集で導入することで内在的な発現条件下でのタンパク質レベルとして測定し、さらにシンプルな生物発光測定法で同じサンプルで細胞内タンパク質をリアルタイムに追跡することができた。Endurazine™ および Vivazine™ substrate は、低発現でも最大の検出感度が得られるよう自家発光バックグラウンドを低減するように設計した。

Kinetically Monitor Endogenous BRD4 Levels

Real time expression of HiBiT-BRD4 fusion was monitored using Endurazine and Vivazine live cell substrates.
HEK293 cells stably expressing LgBiT were modified via CRISPR-Cas9 to create cells expressing a HiBiT-BRD4 fusion at the endogenous BRD4 locus. Expression levels of a HiBiT-BRD4 fusion were monitored in real time using Endurazine™ and Vivazine® substrates following treatment with ARV-771, a BRD4-targeted protein degrader (PROTAC).

NanoLuc® 転写レポーター活性の時間的変化の測定

Endurazine™ および Vivazine™ substrate は、長時間にわたり同じサンプルで転写応答ダイナミックスを測定するために使用する不安定化 NanoLuc® レポーター (NanoLuc-Pest, NlucP) を用いた生細胞アッセイに使用することができます。

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HEK293 cells were transiently transfected with a construct expressing NlucP from a promoter with cAMP response elements (CRE). Endurazine™ and Vivazine® substrates were added to cells at time zero, and luminescence was measured continuously using a GloMax® Discover instrument at 37°C. After approximately 2 hours, varying concentrations of forskolin were added to induce expression of NlucP by increasing the intracellular cAMP concentration. Panel A. Ten hour time course using Endurazine™ substrate. Panel B. Ten hour time course using Vivazine® substrate. Panel C. Per-well normalization for the data in Panel A. Panel D. Per-well normalization for the data in Panel B. Both substrates detect a dose-dependent increase of CRE reporter activity in response to increasing cAMP levels. The Vivazine® substrate provides higher overall signal.

Get Faster Live-cell Kinetic Results with the LgBiT mRNA Delivery System

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HiBiT signal peaks earlier after transfection with the ViaScript® LgBiT mRNA Delivery System than with DNA plasmid transfection. This allows you to start your experiments sooner and get data faster.

Transfection with LgBiT mRNA results in a stable signal over 48 hours. Expand your kinetic workflow by pairing a CRISPR HiBiT cell line and the ViaScript® LgBiT mRNA Delivery System to get more representative data.

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製品仕様

You are viewing: N2570 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Endurazine™ Substrate, 100X

N257A 1 × 0.1ml

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

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パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,836,767 and other patents pending.

製品仕様

You are viewing: N2571 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Endurazine™ Substrate, 100X

N257B 1 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,836,767 and other patents pending.

製品仕様

You are viewing: N2572 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Endurazine™ Substrate, 100X

N257B 10 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,836,767 and other patents pending.

製品仕様

You are viewing: N2580 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Vivazine® Substrate, 100X

N258A 1 × 0.1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,836,767 and other patents pending.

製品仕様

You are viewing: N2581 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Vivazine® Substrate, 100X

N258B 1 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,836,767 and other patents pending.

製品仕様

You are viewing: N2582 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Vivazine® Substrate, 100X

N258B 10 × 1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,836,767 and other patents pending.

製品仕様

You are viewing: N2590 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Endurazine™ Substrate, 100X

N257A 1 × 0.1ml

Nano-Glo® Vivazine® Substrate, 100X

N258A 1 × 0.1ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 10,836,767 and other patents pending.

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