Nano-Glo® Live Cell Assay System

Part Numbers: N2011, N2012, N2013

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生きた細胞でNanoLuc®の発光測定

  • 安定性を向上させるために最適化された基質とバッファー
  • NanoBiT®タンパク質相補アッセイまたは NanoLuc®レポーター活性の検出に
  • 細胞生存性を損なわずに、1つのタイムポイントあるいは連続での発光モニタリングが最長2時間まで可能

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選択製品のカタログ番号: N2011

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Nano-Glo® Live Cell Assay System
100 assays
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Single-Addition Reagent to Detect NanoLuc® Luminescence in Live Cells

Nano-Glo® Live Cell Assay System は生細胞よりNanoBiT™ または NanoLuc® の発光を測定する1液添加方式の非細胞溶解性の試薬です。Nano-Glo® Live Cell SubstrateをNano-Glo® LCS Dilution Bufferで希釈してNano-Glo® Live Cell Reagent, a 5X stockを調製し、培地中に直接添加します。基質とバッファーとも安定性を向上させるために最適化されています。 Nano-Glo® Live Cell Reagent は血清の有無にかかわらず自己発光を抑えるようにデザインされており、低レベルのNanoBiT™ または NanoLuc® を検出できるよう感度を上げています。Nano-Glo® Live Cell Assay System は細胞生存性を損なわずに最長2時間までの設定された時点あるいは連続での発光モニタリングに使用することができます。

NanoLuc® technologyの詳細についてはこちら

NanoBiT™ technologyの詳細についてはこちら

【ライセンス】NanoGlo

Cat.# N2011 is sufficient for 100 assays in 96-well plates; Cat.# N2012 is sufficient for 1,000 assays in 96-well plates, Cat.# N2013 is sufficient for 10,000 assays in 96-well plates.

The signal stability observed after addition of the Nano-Glo® Live Cell Reagent to culture media can be influenced by experimental parameters such as high levels of enzyme activity, temperature or the presence of FBS in the media. Please refer to #TM461 for additional information.

Signal stability of the Nano-Glo® Live Cell Reagent in HEK293 cells expressing varying levels of NanoLuc® Luciferase.
Signal stability of the Nano-Glo® Live Cell Reagent in HEK293 cells expressing varying levels of NanoLuc® Luciferase.

製品仕様

You are viewing: N2011 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Live Cell Substrate

N205A 1 × 125μl

Nano-Glo® LCS Dilution Buffer

N206A 1 × 2.5ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. No. 9,676,997, European Pat. No. 3146066, Japanese Pat. No. 6641297 and other patents and patents pending.

製品仕様

You are viewing: N2012 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Live Cell Substrate

N205B 1 × 1.25ml

Nano-Glo® LCS Dilution Buffer

N206B 1 × 25ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. No. 9,676,997, European Pat. No. 3146066, Japanese Pat. No. 6641297 and other patents and patents pending.

製品仕様

You are viewing: N2013 Change Configuration

選択製品の構成品内容

Item Part # Size

Nano-Glo® Live Cell Substrate

N205B 10 × 1.25ml

Nano-Glo® LCS Dilution Buffer

N206C 5 × 50ml

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

See Protocol for detailed storage recommendations.

パテントおよび免責条項

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.

For uses of Nano-Glo®-branded reagents intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(i) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays to energy acceptors not manufactured by Promega.

With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. No. 9,676,997, European Pat. No. 3146066, Japanese Pat. No. 6641297 and other patents and patents pending.

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