FuGENE® SI Transfection Reagent
Versatile Transfection Reagent for siRNA, miRNA and Other Small RNA Molecules
- High-efficiency transfection in both challenging and routine eukaryotic cell lines
- Maximum knockdown with low toxicity—fewer cells required
- Simple protocol with minimal optimization
Catalog Number:
Size
High-Efficiency, Gentle Reagent for Small RNA Delivery
FuGENE® SI is an innovative, 100% synthetic, multicomponent transfection reagent designed for the delivery of siRNA, miRNA and other small RNA molecules. FuGENE® SI provides high-efficiency delivery of RNA into a wide range of eukaryotic cell lines while providing the gentle, low-toxicity transfection you expect from FuGENE® reagents. Obtain maximum knockdown efficiencies while minimizing impact on cell health, allowing you to get the most out of transfection-based experiments.
Animal-free reagents and strong performance make FuGENE® SI ideal for a variety of RNA transfection applications from functional genomics to siRNA library screens.
The quick and easy protocol allows you to obtain superior results with minimal optimization, freeing-up valuable lab time.
Gentle and Nontoxic siRNA Transfection
FuGENE® SI Transfection Reagent delivers gentle and nontoxic siRNA transfection. HEK293-GFP and NIH3T3-GFP cell lines (Cell Biolabs) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0 or 5pmol of GFP targeting siRNA or negative control (Dharmacon™, Horizon) along with 0.3µl of FuGENE® SI Transfection Reagent. Cells were then analyzed via flow cytometry 48 hours post-transfection to measure total cell viability. Graphs show the gentle and nontoxic transfection of siRNAs using FuGENE® SI.
Superior Knockdown Efficiency
FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency. HEK293-GFP and NIH3T3-GFP cell lines (Cell Biolabs) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0 or 5pmol of GFP targeting siRNA or negative control (Dharmacon™, Horizon) along with 0.3µl of FuGENE® SI Transfection Reagent or 0.3µl of Lipofectamine™ RNAiMAX (Life Technologies). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % knockdown of GFP and total cell viability. Graphs show the superior knockdown performance of FuGENE® SI versus Lipofectamine™ RNAiMAX in HEK293 and NIH3T3 cells.
FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency, as confirmed by Western blot. Human MDA-MB-468 cells were reverse-transfected in 6-well plates with 40pmol of the indicated targeting siRNAs or negative/positive control (Silencer® Select siRNA, Invitrogen) utilizing either 7.5µl of FuGENE® SI Transfection Reagent or 7.5µl of Lipofectamine™ RNAiMAX per well. The cells were collected 72 hours after transfection and analyzed via Western blot for expression of target genes STAT3 and CEBPB. The Western blot shown here demonstrates the robust, superior knockdown with FuGENE® SI as compared to the market leading competitor.
Efficient Transfection of Labeled Small RNAs
FuGENE® SI provides efficient transfection of labeled small RNAs. A549, COS-7, HEK293 and HeLa cells plated in 96-well microplates were transfected with 0.4µl of FuGENE® SI or a no-reagent negative control and various amounts of FITC-labeled negative control siRNA. Twenty-four hours after transfection, the cells were analyzed via flow cytometry for FITC-positive signal.
Protocols
Complete Protocol
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
FuGENE® SI Transfection Reagent |
E931A | 1 × 1ml |
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
FuGENE® SI Transfection Reagent |
E931A | 5 × 1ml |
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