VA1160_rAsp-N-Mass-Spec-Grade

Hydrolyzes Peptide Bonds N-Terminal to Aspartic Acid Residues

  • Less expensive alternative to native Asp-N
  • Larger volume (5X more protease) for more consistent resuspension
  • Use in complex proteomic analyses and peptide mapping of purified proteins

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Catalog Number: VA1160

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Overview
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Specifications
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Asp-N の組換え型がご利用可能になりました!

rAsp-N, Mass Spec Grade は Stenotrophomonas maltophilia よりクローニングされ、大腸菌より精製された組換え型プロテアーゼです。rAsp-N は非常に活性が高く、複雑な混合液からのプロテオーム分析や治療用モノクローナル抗体などの精製タンパク質のペプチドマッピングなどに適しています。プロテアーゼは 円すい型のバイアルへ10µg ごとに分注されており、簡便で懸濁も容易です。

1時間以内で終了する効率的なタンパク質消化

Images shows rAsp-N digests N-terminal to aspartic acid residues.
Recombinant Asp-N provides efficient digestion of a yeast extract in 1 hour. Digestion efficiency was approximately 85% after 1 hour and increased only slightly (to approximately 91%) after overnight digestion.

アスパラギン酸残基のN末端側を消化

Image shows digestion efficiency of rAsp-N.
rAsp-N, Mass Spec Grade, digests yeast extract proteins primarily N-terminal to aspartic acid residues. Weak digestion at glutamic acid is observed in some cases.

rAsp-N または Trypsin/Lys-C によるペプチドマッピング

Peptide mapping with rAsp-N and Trypsin/Lys-C.
Base peak chromatograms showing digestion of NISTmAb for 18 hours with rAsp-N (Panel A; 50:1 w:w) or with Trypsin/Lys-C (Panel B; 25:1 w:w). Digests were analyzed with a Q Exactive™ Plus mass spectrometer (Thermo Fisher Scientific). Sequence coverage from each digest is shown in the table (Panel C).

Specifications

Catalog Number:

選択製品の構成品内容

Item Part # Size

rAsp-N, Mass Spec Grade

VA116A 1 × 10μg

分析証明書

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使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

BB

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