Rapid-Digestion Trypsin and Rapid-Digestion Trypsin/Lys-C Kits
Minimize Protein Digestion Time, Maximize Results
- Faster digestion time
- Streamlined workflow
- Tighter coefficients of variation
Catalog Number:
Choose a Kit
Size
Catalog Number: VA1060
Catalog Number: VA1061
わずか60分に消化時間を短縮
Rapid Digestion-Trypsin および Rapid Digestion-Trypsin/Lys-C Kit は通常 4～18時間かかるタンパク質消化を 60 分間にまで短縮するためにデザインされています。どちらのキットも迅速消化に最適化された i) プロテアーゼ (Trypsin または Trypsin/Lys-C Mix); ii) protease Resuspension Buffer; および iii) Rapid Digestion Buffer の3つで構成されています．
これらのキットを用いたタンパク質消化は、迅速で効率的な分かりやすいプロトコルで行われます。様々なサンプル量およびタンパク質濃度で実施できる柔軟性のあるプロトコルで、特殊な機器やオフラインでの脱塩は不要です。サンプル調製の全工程はわずか 60 分間で完了します。
Simple Process Enables Multiomic Analyses from a Common Cell Culture Sample
The reach of omic analyses now includes the breadth of the cell life cycle, allowing study of processes from translation of RNA to protein, through proteome development and interactions, and metabolic activities. However, as Caprioli et al. (2018) note:
"Many published methodologies for preparing samples for proteomic analysis by mass spectrometry are entirely incompatible with the analysis of metabolites or RNA from the same sample due to the presence of specific buffer components or detergents."
The ideal, to study multiple omic systems from a single sample, is now possible using the process described in this Journal of Proteome Research paper: “An Integrated, High-Throughput Strategy for Multiomic Systems Level Analysis".
In the paper Caprioli et al. used Rapid-Digestion Trypsin/Lys-C (described on this page) in their tryptic digestion of proteomic samples.
標準的な消化時間よりもずっと短時間で完了するトリプシン消化
ペプチドスペクトルマッチング の比較：Rapid Digestion–Trypsin vs 標準消化
The increase in spectra noted for Rapid-Digestion Trypsin sample preparation (30-minute digestion) indicates more complete digestion than standard overnight digestion (16 hours using Trypsin Gold, Mass Spectrometry Grade).
還元・アルキル化によるシーケンスカバレッジの改善
Both Rapid-Digestion Trypsin and Rapid-Digestion Trypsin/Lys-C (35-minute digestions) are compatible with reduction and alkylation steps, resulting in increased sequence coverage.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
Resuspension Buffer |
V181A | 1 × 500μl |
Rapid Trypsin Gold, MS Grade |
V528B | 1 × 100μg |
Rapid Digest Buffer |
VA106A | 1 × 30ml |
選択製品の構成品内容
| Item | Part # | Size |
|---|---|---|
|
Resuspension Buffer |
V181A | 1 × 500μl |
Rapid Trypsin/Lys-C Mix, MS Grade |
V507C | 1 × 100μg |
Rapid Digest Buffer |
VA106A | 1 × 30ml |
Resources
No related resources found
Related Products
類似製品
Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin GoldとrLys-Cの混合物で高いペプチド回収率、優れたタンパク質定量精度、安定した再現性
V5071, V5072, V5073
Sequencing Grade Modified Trypsin
Highly active and stable trypsin manufactured to provide maximum specificity.
V5111, V5117, V5113
Trypsin Gold, Mass Spectrometry Grade
Maximum digest specificity with extreme resistance to autolytic digestion.
V5280
関連製品
Magnetic Proteomics Sample Prep (MPSP) Kit
SP3法によるプロテオームサンプル調製を効率化する磁気ビーズ
CS3325A04
IdeS and IdeZ Proteases
IgGのヒンジ領域の下を一か所切断する酵素
V7511, V7515, V8341, V8345
PNGase F
N結合型糖タンパク質から高マンノース、ハイブリッド型オリゴ糖、複合型オリゴ糖の最も内側のGlucNAc残基とアスパラギン残基間のN結合型糖切断を触媒します
V4831
Asp-N, Sequencing Grade
アスパラギン酸 (Asp)とシステイン酸 (Cys)のＮ末端側のペプチド結合を加水分解するエンドプロテイナーゼ
V1621
