ReliaPrep™ RNA Clean-Up and Concentration System
Concentrate RNA from Large Volumes to ≤15µl
- Column-based method for rapid concentration and clean-up of RNA
- High binding capacity to accommodate large samples
- Small elution volumes provide highly concentrated samples and remove inhibitors
Catalog Number:
Size
Catalog Number: Z1071
Catalog Number: Z1072
Catalog Number: Z1073
Prepare RNA Samples for Downstream Applications
下流のアプリケーションのためのRNA調製
ReliaPrep™ RNA Clean-Up and Concentration System は試薬のキャリーオーバーを低減し、濃度の低いサンプルをおどろくほど簡便に濃縮するためにデザインされています。このシステムでは1本のカラムで最大 300µl の濃縮前サンプルを処理でき、水またはTE バッファー 15µl に溶出することができます。RNAの回収率も高く、RT-qPCR、ノーザンブロッティングあるいはRNA シークエンシングなどの用途に使用することができます。
ReliaPrep™ RNA Clean-Up and Concentration System による低濃度溶液からの回収と濃縮
Five micrograms of mouse liver RNA was suspended in 100, 150, 200 or 300µl of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Results shown demonstrate equivalent yield regardless of target RNA concentration. Average RNA yield was 79.8%.
One microgram of mouse liver RNA was suspended in 100, 150, 200 or 300µl of water, repurified using the standard protocol and eluted in 15µl of Nuclease-Free Water. Results shown demonstrate equivalent yield regardless of target RNA concentration. Average RNA yield was 74.9%.
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 1 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
|
Membrane Binding Solution |
A930A | 1 × 4ml | View Product |
|
Elution Tubes |
Z200A | 2 × 5/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
Membrane Binding Solution |
A930B | 1 × 20ml | View Product |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
ReliaPrep™ Minicolumns |
A207B | 1 × 50/pack | |
Collection Tubes |
A130A | 1 × 50/pk | |
|
Elution Tubes |
Z200B | 2 × 25/pk | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
|
Membrane Binding Solution |
A930B | 2 × 20ml | View Product |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z3091 | 1 × 58.8ml | View Product |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
Collection Tubes |
A130B | 1 × 250/pk | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
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