ReliaPrep™ FFPE gDNA Miniprep System
Intact, Amplifiable DNA from FFPE Tissue
- Minimal preparation time; simply add ethanol and go!
- Safely deparaffinize without using harsh organic solvents
- No overnight digestion required
Catalog Number:
Size
Catalog Number: A2351
Catalog Number: A2352
FFPEサンプルより良質なDNAを抽出
ReliaPrep™ FFPE Miniprep Systems Help Preserve DNA Integrity
In this experiment, 10µl of gDNA purified from FFPE human liver samples was used as template in multiplex PCR to amplify fragments of the GAPDH gene. DNA fragments ranging from 100–400bp were detected.
高感度なアッセイに適したFFPE DNA を精製
ゲノム DNA はヒト肝臓 FFPE 切片 10µm より ReliaPrep™ FFPE gDNA Miniprep System を用いてせ精製し、Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641) を用いて STR 分析を行った。
シンプルなプロトコール
Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Collection Tubes |
A130C | 1 × 10/pk | ||
|
Mineral Oil |
A244A | 1 × 5ml | ||
|
ReliaPrep™ FFPE Binding Columns |
A247A | 1 × 10/pk | ||
|
Proteinase K (PK) Solution |
A505A | 1 × 250μl | View Product | |
|
RNase A Solution |
A797D | 1 × 200μl | 4mg/ml | View Product |
|
Elution Buffer |
A828C | 1 × 15ml | View Product | |
|
Lysis Buffer (LBA) |
Z101A | 2 × 1ml | ||
|
BL Buffer |
Z103A | 1 × 3.25ml | ||
|
Wash Solution |
Z104A | 1 × 3ml | ||
Blue Dye |
MC122A | 1 × 100μl |
選択製品の構成品内容
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | ||
|
Mineral Oil |
A244B | 1 × 50ml | ||
|
ReliaPrep™ FFPE Binding Columns |
A247B | 2 × 50/pk | ||
|
Proteinase K (PK) Solution |
A505B | 2 × 1.1ml | View Product | |
|
RNase A Solution |
A797C | 1 × 1ml | 4mg/ml | View Product |
|
Elution Buffer |
A828C | 1 × 15ml | View Product | |
|
Lysis Buffer (LBA) |
Z101B | 1 × 30ml | ||
|
BL Buffer |
Z103B | 1 × 32.5ml | ||
|
Wash Solution |
Z104B | 1 × 30ml | ||
Blue Dye |
MC122A | 1 × 100μl |
Resources
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