Wizard® SV Genomic DNA Purification System

Peripheral blood mononuclear cells

Yes

Ezzeldin, H.H. et al. (2005) Clin. Cancer Res. 11, 8699–705.
PubMed ID #16361556.

Mouse primary granulocytes, macrophages, B lymphocytes and T lymphocytes

Yes

Morii, E. et al. (2004) Blood 104, 1656–61.
PubMed ID #15172970.

Mouse liver

Yes

A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.

Mouse brain

Yes

A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.

Mouse heart

Yes

A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.

Mouse spleen

Yes

Fakhari, F.D. et al. (2006) J. Clin. Invest. 116, 735–42.
PubMed ID #16498502.

Mouse ear

Yes

Du, Q. et al. (2006) FASEB J. 20, 1131–41.
PubMed ID #16770012.

Mouse skin

Yes

Basal cell carcinoma and noncancerous tissues were frozen at –80°C prior to DNA isolation.
Mancuso, M. et al. (2006) Cancer Res. 66, 6606–14.
PubMed ID #16818633.

Anopheles funestus mosquito carcass

Yes

Michel, A.P. et al. (2006) Genetics 173, 1389–95.
PubMed ID #16648581.

Human lung cancer biopsy

Yes

Sasaki, H. et al. (2005) Clin. Cancer Res. 11, 2924–9.
PubMed ID #15837743.

Human distant normal colorectal mucosa and tumor tissue

Yes

Pfeifer, D. et al. (2005) Carcinogenesis 26, 103–7.
PubMed ID #15485994.

Human frozen thymic epithelial tumor samples

Yes

Suzuki, E. et al. (2006) Jpn. J. Clin. Oncol. 36, 351–6.
PubMed ID #16762968.

Human pancreatic islet

Yes

Del Guerra, S. et al. (2005) Diabetes 54, 727–35.
PubMed ID #15734849.

Chicken retinospheroid cultures

Yes

Paraoanu, L.E. et al. (2006) Toxicol. Sci. 89, 314–24.
PubMed ID #16207942.

HT1080 human fibrosarcoma

Yes

Cells were transfected with FIV vectors, and genomic DNA was isolated 3 days post-transduction.
Harper, S.Q. et al. (2006) J. Virol. 80, 9371–80.
PubMed ID #16973543.

Monkey primary fibroblasts, virus-infected

Yes

Cells were infected with ~1 multiplicity of infection (MOI) of pseudotyped HCV and total DNA isolated.
Blanchard, E. et al. (2006) J. Virol. 80, 6964–72.
PubMed ID #16809302.

E. coli K-12 derivative, MC4100

Yes

Teresa Pellicer, M. et al. (2003) J. Bacteriol. 185, 5815–21.
PubMed ID #13129953.

Pseudomonas aeruginosa

Yes

Zhu, H. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45, 1897–903.
PubMed ID #15161855.

Bacillus subtilis

Yes

Park, J.H. et al. (2004) J. Bacteriol. 186, 1571–3.
PubMed ID #14973012.

Eleven Gram-negative bacterial species

Yes

Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2 mg/ml proteinase K was added (final concentration 0.4 mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9.
PubMed ID #16891488.

Eleven Gram-positive bacterial species

Yes

Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2mg/ml proteinase K was added (final concentration 0.4mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9.
PubMed ID #16891488.

Snail cercariae

Yes

Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716.