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PureYield™ Plasmid Midiprep System

Part Numbers: A2492, A2495, A2496

PureYield Plasmid Midiprep System 100 preps
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50ml の培養液から最大 200µg のトランスフェクション品質のプラスミドを精製

  • 30 分間の吸引法プロトコル
  • 高純度の濃縮されたプラスミド DNA
  • 時間のかかる重力滴下カラムや溶出後のアルコール沈殿は不要

Size

選択製品のカタログ番号: A2492

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This product is discontinued
This product is available under our Early Access program - Learn More
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PureYield™ Plasmid Midiprep System
25 preps
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Change Configuration

 PureYield™ Plasmid Midiprep Systemにより、50mlの大腸菌培養液から100-200μgのトランスフェクション品質のプラスミドDNAを迅速な方法で精製することができます。吸引プロトコルにより、プラスミド精製は30分間で完了し、シリカ樹脂や他のメンブランベースの精製法に比べ大幅に操作時間を短縮することができます。もう一つのプロトコルにより、250mlの培養液からコピー数の高いプラスミドであれば400μg以上精製を行うこともできます。

このシステムには、大部分のタンパク質、RNAおよびエンドトキシンを精製プラスミドDNAからを除去するユニークなEndotoxin Removal Washが含まれています。夾雑物の除去により、真核生物へのトランスフェクション、in vitro 転写、in vitro 転写翻訳共役系などの影響の受けやすい系で常に良い結果が得られます。このシステムでは、イソプロパノール沈殿や遠心分離が不要なプラスミドDNA精製システムです。

PureYield™ Plasmid Midiprepは、遠心法あるいは吸引法を選んで使うことができます。

Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield Plasmid Midiprep System DNA Purification by Vacuum protocol.

プロトコール

Complete Protocol

Quick Protocols

製品仕様

You are viewing: A2492 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Neutralization Solution (NSB)

 A148H 1 × 130ml View Product

PureYield™ Binding Columns

 A245A 5 × 5 each

PureYield™ Clearing Columns

 A246A 5 × 5 each

Endotoxin Removal Wash

 A251C 1 × 85.3ml

Column Wash

 A252C 1 × 210ml

Cell Resuspension Solution (CRA)

 A711F 1 × 75ml View Product

Cell Lysis Solution (CLA)

 A712F 1 × 75ml View Product

Nuclease-Free Water

 P119C 1 × 25ml View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

Store all system components at 22–25°C.

製品仕様

You are viewing: A2495 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Neutralization Solution (NSB)

 A1485 1 × 500ml

PureYield™ Binding Columns

 A245A 20 × 5 each

PureYield™ Clearing Columns

 A246A 20 × 5 each

Endotoxin Removal Wash

 A251B 1 × 315ml

Column Wash

 A252B 2 × 381ml

Cell Resuspension Solution (CRA)

 A7115 1 × 315ml

Cell Lysis Solution (CLA)

 A7125 1 × 315ml

Nuclease-Free Water

 P119C 4 × 25ml View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

Store all system components at 22–25°C.

製品仕様

You are viewing: A2496 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Neutralization Solution (NSB)

 A1485 3 × 500ml

PureYield™ Binding Columns

 A245E 3 × 100 each

PureYield™ Clearing Columns

 A246E 3 × 100 each

Endotoxin Removal Wash

 A251B 3 × 315ml

Column Wash

 A252B 6 × 381ml

Cell Resuspension Solution (CRA)

 A7115 3 × 315ml

Cell Lysis Solution (CLA)

 A7125 3 × 315ml

Nuclease-Free Water

 P1195 2 × 150ml View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

Store all system components at 22–25°C.

技術資料

サイテーション

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A1071

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実績豊富、信頼のパフォーマンスで一般的な細胞株に最適

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ViaFect™ Transfection Reagent

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