Wizard® Plus SV Minipreps DNA Purification Systems
1–20μg High-Quality Plasmid DNA from 1–10ml Cultures
- Process up to 20 minipreps in 45 minutes
- Choice of spin or vacuum purification formats
- No phenol extractions or precipitations required
Catalog Number:
Size
Additional options
Catalog Number: A1330
Catalog Number: A1460
Catalog Number: A1465
Catalog Number: A1340
Catalog Number: A1470
Wizard® Plus SV Minipreps DNA Purification Systemはシリカメンブランを使ったシステムで、迅速なプラスミドDNA精製のシンプルで再現性のある方法です。サンプル数にもよりますが、操作は45分間かそれ以下です。大腸菌のオーバーナイト培養液1-10mlからプラスミド精製が行えます。BigDye®ターミネーターによるpGEM®-3Zf(+)ベクターのシークエンシングで98%を超える精度で500塩基以上解析できるという条件を満たす精製プラスミドDNAが得られます。また、リボヌクレアーゼインヒビター、例えばRNasin® (Cat.# N2511)を添加して、in vitro 転写系のテンプレートとして用いることもできます。
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129A | 1 × 50/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
|
Column Wash Solution (CWA) |
A131B | 1 × 20ml | View Product |
|
Alkaline Protease Solution |
A144A | 1 × 550μl | View Product |
|
Neutralization Solution (NSB) |
A148B | 1 × 30ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711P | 1 × 20ml | View Product |
|
Cell Lysis Solution (CLA) |
A712P | 1 × 20ml | View Product |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 1 × 250/pk | |
|
Collection Tubes |
A130B | 1 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 1 × 100ml | View Product |
|
Alkaline Protease Solution |
A144B | 1 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 1 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 1 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 1 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 4 × 250/pk | |
|
Collection Tubes |
A130B | 4 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 4 × 100ml | View Product |
|
Alkaline Protease Solution |
A144B | 4 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 4 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 4 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 4 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 2 × 25ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129A | 1 × 50/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
|
Column Wash Solution (CWA) |
A131B | 1 × 20ml | View Product |
|
Vacuum Adapters |
A1331 | 1 × 20 each | |
|
Alkaline Protease Solution |
A144A | 1 × 550μl | View Product |
|
Neutralization Solution (NSB) |
A148B | 1 × 30ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711P | 1 × 20ml | View Product |
|
Cell Lysis Solution (CLA) |
A712P | 1 × 20ml | View Product |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 1 × 250/pk | |
|
Collection Tubes |
A130B | 1 × 250/pk | |
|
Column Wash Solution (CWA) |
A131C | 1 × 100ml | View Product |
|
Vacuum Adapters |
A1331 | 1 × 20 each | |
|
Alkaline Protease Solution |
A144B | 1 × 3ml | View Product |
|
Neutralization Solution (NSB) |
A148C | 1 × 100ml | View Product |
|
Cell Resuspension Solution (CRA) |
A711N | 1 × 75ml | View Product |
|
Cell Lysis Solution (CLA) |
A712N | 1 × 75ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
Resources
その他
Related Products
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