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Wizard® Plus SV Minipreps DNA Purification Systems

Part Numbers: A1330, A1460, A1465, A1340, A1470

Wizard Plus SV Minipreps DNA Purif System 50 preps
Wizard Plus SV Minipreps DNA Purif System 250 preps
Wizard Plus SV Minipreps DNA Purif System 50 preps
customize-this-small

培養液1 - 10ml から高品質プラスミド DNA 1 - 20μg を精製

  • 最大 20 のミニプレップを45分間に処理
  • スピン法あるいは吸引法精製フォーマットを選択可能
  • フェノール抽出またはエタノール沈殿は不要

Size

Additional options

選択製品のカタログ番号: A1330

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Wizard® Plus SV Minipreps DNA Purification Systems
50 preps
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Change Configuration

Wizard® Plus SV Minipreps DNA Purification Systemはシリカメンブランを使ったシステムで、迅速なプラスミドDNA精製のシンプルで再現性のある方法です。サンプル数にもよりますが、操作は45分間かそれ以下です。大腸菌のオーバーナイト培養液1-10mlからプラスミド精製が行えます。BigDye®ターミネーターによるpGEM®-3Zf(+)ベクターのシークエンシングで98%を超える精度で500塩基以上解析できるという条件を満たす精製プラスミドDNAが得られます。また、リボヌクレアーゼインヒビター、例えばRNasin® (Cat.# N2511)を添加して、in vitro 転写系のテンプレートとして用いることもできます。

プロトコール

Complete Protocol

Quick Protocols

製品仕様

You are viewing: A1330 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Wizard® SV Minicolumns

 A129A 1 × 50/pk

Collection Tubes

 A130A 1 × 50/pk

Column Wash Solution (CWA)

 A131B 1 × 20ml View Product

Alkaline Protease Solution

 A144A 1 × 550μl View Product

Neutralization Solution (NSB)

 A148B 1 × 30ml View Product

Cell Resuspension Solution (CRA)

 A711P 1 × 20ml View Product

Cell Lysis Solution (CLA)

 A712P 1 × 20ml View Product

Nuclease-Free Water

 P119E 1 × 13ml View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

製品仕様

You are viewing: A1460 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Wizard® SV Minicolumns

 A129B 1 × 250/pk

Collection Tubes

 A130B 1 × 250/pk

Column Wash Solution (CWA)

 A131C 1 × 100ml View Product

Alkaline Protease Solution

 A144B 1 × 3ml View Product

Neutralization Solution (NSB)

 A148C 1 × 100ml View Product

Cell Resuspension Solution (CRA)

 A711N 1 × 75ml View Product

Cell Lysis Solution (CLA)

 A712N 1 × 75ml View Product

Nuclease-Free Water

 P119C 1 × 25ml View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

製品仕様

You are viewing: A1465 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Wizard® SV Minicolumns

 A129B 4 × 250/pk

Collection Tubes

 A130B 4 × 250/pk

Column Wash Solution (CWA)

 A131C 4 × 100ml View Product

Alkaline Protease Solution

 A144B 4 × 3ml View Product

Neutralization Solution (NSB)

 A148C 4 × 100ml View Product

Cell Resuspension Solution (CRA)

 A711N 4 × 75ml View Product

Cell Lysis Solution (CLA)

 A712N 4 × 75ml View Product

Nuclease-Free Water

 P119C 2 × 25ml View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

製品仕様

You are viewing: A1340 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Wizard® SV Minicolumns

 A129A 1 × 50/pk

Collection Tubes

 A130A 1 × 50/pk

Column Wash Solution (CWA)

 A131B 1 × 20ml View Product

Vacuum Adapters

 A1331 1 × 20 each

Alkaline Protease Solution

 A144A 1 × 550μl View Product

Neutralization Solution (NSB)

 A148B 1 × 30ml View Product

Cell Resuspension Solution (CRA)

 A711P 1 × 20ml View Product

Cell Lysis Solution (CLA)

 A712P 1 × 20ml View Product

Nuclease-Free Water

 P119E 1 × 13ml View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

製品仕様

You are viewing: A1470 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Wizard® SV Minicolumns

 A129B 1 × 250/pk

Collection Tubes

 A130B 1 × 250/pk

Column Wash Solution (CWA)

 A131C 1 × 100ml View Product

Vacuum Adapters

 A1331 1 × 20 each

Alkaline Protease Solution

 A144B 1 × 3ml View Product

Neutralization Solution (NSB)

 A148C 1 × 100ml View Product

Cell Resuspension Solution (CRA)

 A711N 1 × 75ml View Product

Cell Lysis Solution (CLA)

 A712N 1 × 75ml View Product

Nuclease-Free Water

 P119C 1 × 25ml View Product

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

技術資料

サイテーション

その他

類似製品

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スピンあるいは吸引法を選べる、マニュアル操作あるいは自動化96フォーマット

A2250, A2255, A2258

PureYield™ Plasmid Miniprep System

10分間で3mlの培養液から最大15μgのトランスフェクション品質のプラスミドDNA

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PureYield™ Plasmid Midiprep System

50ml培養液から30分でトランスフェクション品質のプラスミドを最大200µg精製

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Wizard® SV Genomic DNA Purification System

細胞、組織あるいはマウス尾からDNAを精製するシンプルなシリカメンブラン法

A2360, A2361

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Column Wash Solution (CWA)

Wizard®, Wizard® SVおよびWizard® SV 96 Genomic DNA Purification Kitのコンポーネントで、別途購入可能

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JM109 Competent Cells

青/白スクリーニングが行えるコンピテントセル

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