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Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems

A2250-wizard-sv-96-plasmid-dna-purification-system A2255-wizard-sv-96-plasmid-dna-purification-system new-kit-box

Silica Membrane-Based, 96-Well, High-Throughput Plasmid Purification

  • 60-minute protocol
  • Manual and automated formats
  • Vacuum-based method

Catalog Number:

Size

Catalog Number: A2250

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Catalog Number: A2255

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Catalog Number: A2258

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Overview
Protocols
Specifications
Resources
Related Products

Simple, Reliable Plasmid Purification

Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systemはシリカ膜を使った、迅速なプラスミドDNA精製のシンプルで再現性のある方法で、96ウェル、ハイスループットフォーマットです。一枚のプレートの処理時間は60分間かそれ以下です。Vac-Man® 96 Vacuum Manifoldを使うことで、排液の取扱いを無くし、ライセートの清澄化とDNAの結合を同時に行うことができ、サンプル処理中のクロスコンタミネーションを防げます。

精製プラスミドは、そのままDNAシークエンシングや一般的な分子生物学手法に用いることができます。BigDye®ターミネーターによるpGEM®-3Zf(+)ベクターのシークエンシングで98%を超える精度で600塩基以上解析できるという条件を満たす精製プラスミドDNAが得られます。

Wizard® SV 96 and SV 9600 Systemは、手作業あるいは自動分注ロボットを用いて行うことができます。評価済みの自動化方法は、www.promega.com/automethods/ をご参照ください。

このシステムには、真空度50-68kPaの真空ポンプ、Vac-Man® 96 Vacuum Manifold (Cat.# A2291)あるいは同等のマニホールドが必要です。Vac-Man® 96 Vacuum Manifoldを用いる場合、真空トラップを別途ご準備下さい。

この製品のカスタムオプションについては、www.promega.com/custom/ をご参照ください。

The Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems require the use of a vacuum manifold (Vac-Man 96 Vacuum Manifold, Cat.# A2291).
The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems require use of a vacuum manifold. (Vac-Man® 96 Vacuum Manifold Cat.# A2291).

Specifications

Catalog Number:

選択製品の構成品内容

Item Part # Size Available Separately

Alkaline Protease Solution

A144B 1 × 3ml View Product

Wizard® SV 96 Neutralization Solution

A148D 1 × 84ml View Product

Wizard® SV 96 Lysate Clearing Plate

A224A 1 × 1 plate View Product

96-Well Deep Well Plate

A226A 1 × 1 plate

Binding Plate

A227A 1 × 1 plate View Product

Elution Plate

A228A 1 × 1 plate

Plate Sealers

A230A 1 × 3 each

Wizard® SV 96 Cell Resuspension Solution

A711U 1 × 40ml View Product

Wizard® SV 96 Cell Lysis Solution

A712U 1 × 40ml View Product

Nuclease-Free Water

P119E 1 × 13ml View Product

Column Wash Solution (CWA)

A131C 1 × 100ml View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Column Wash Solution (CWA)

A1318 1 × 370ml View Product

Alkaline Protease Solution

A144B 2 × 3ml View Product

Wizard® SV 96 Neutralization Solution

A148E 1 × 425ml View Product

Wizard® SV 96 Lysate Clearing Plate

A224A 5 × 1 plate View Product

96-Well Deep Well Plate

A226A 5 × 1 plate

Binding Plate

A227A 5 × 1 plate View Product

Elution Plate

A228A 5 × 1 plate

Plate Sealers

A230A 5 × 3 each

Wizard® SV 96 Cell Resuspension Solution

A711W 1 × 125ml View Product

Wizard® SV 96 Cell Lysis Solution

A712W 1 × 125ml View Product

Nuclease-Free Water

P119C 3 × 25ml View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

選択製品の構成品内容

Item Part # Size Available Separately

Column Wash Solution (CWA)

A1318 20 × 370ml View Product

Wizard® SV 96 Neutralization Solution

A1488 9 × 950ml View Product

Wizard® SV 96 Cell Resuspension Solution

A7118 4 × 800ml View Product

Wizard® SV 96 Cell Lysis Solution

A7128 4 × 800ml View Product

Wizard® SV 96 Lysate Clearing Plates

A2248 1 × 100 pack View Product

Wizard® SV 96 Binding Plates

A2278 1 × 100 pack View Product

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

DD

Resources

その他