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PolyATtract® mRNA Isolation Systems

Part Numbers: Z5210, Z5200, Z5300, Z5310

Z5210_PolyATtract--mRNA-Isolation-System-I_3
Z5300_PolyATtract--mRNA-Isolation-System-III_3
Z5210_PolyATtract--mRNA-Isolation-System-I_3

Total RNA からの 高純度な mRNA 精製

  • 約 45 分でプロトコールが完了
  • in in vitro 転写、RT-PCR および cDNA 合成での使用に適した mRNA を精製
  • Cat.# Z5200 および Z5210 には total RNA 1-5mg からの mRNA 精製 3回分に必要な試薬を含みます。
  • Cat.# Z5300 および Z5310 には total RNA 100-1,000µg からの mRNA 精製 15 回分に必要な試薬を含みます。

Size

Add a magnetic stand

選択製品のカタログ番号: Z5210

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This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
PolyATtract® mRNA Isolation Systems
3 isolations
Your price:
Change Configuration
プロメガのMagneSphere®Streptavidin Paramagnetic Particles(PMPs：ストレプトアビジンコート金属ビーズ)を用いて、迅速かつ効果的にTotal RNAからmRNAを分離するシステムです。操作は3つのステップから成り、短時間で全操作が完了します。1)ビオチン化oligo(dT)とmRNAを溶液中でアニーリング。2)PMPsを用いてハイブリダイズ分子を捕捉。3)Magnetic Separation Standを用いて磁気的にビーズ結合体を回収。得られたmRNAは、in vitro翻訳やRT-PCR,cDNA合成,その他の分子生物学的手法に最適です。Systems IとIIは、1～5mgのTotal RNAからの分離に使用します。Systems IIIとIVは、100～1,000µgのTotal RNAからの分離に使用します。
  • 磁気の利用により、迅速なmRNA分離が実現
  • 未分解のmRNAを高回収率で45分以内に回収
  • 多量あるいは少量のTotal RNAに応じ、2種類のシステムを選択可能

【製品マニュアル（System I & II）】日本語   【製品マニュアル（System III & IV）】日本語

【アプリケーション】PolyATtract® mRNA Isolation Reagents を使った Poly(A)+ mRNA の自動化ロボットによる単離

プロトコール

Complete Protocol

Quick Protocols

製品仕様

You are viewing: Z5210 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Nuclease-Free Water

 P119C 3 × 25ml View Product

SSC, 20X

 Z525A 3 × 1.4ml

Biotinylated Oligo(dT) Probe

 Z526A 1 × 35μl View Product

mRNA User Tube

 Z528A 3 × 1 each

Streptavidin MagneSphere® Paramagnetic Particles

 Z548F 3 × 3ml

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

CC

製品仕様

You are viewing: Z5200 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Nuclease-Free Water

 P119C 3 × 25ml View Product

SSC, 20X

 Z525A 3 × 1.4ml

Biotinylated Oligo(dT) Probe

 Z526A 1 × 35μl View Product

mRNA User Tube

 Z528A 3 × 1 each

MagneSphere® Technology Magnetic Separation Stand (two-position)

 Z5333 1 × 12 × 75mm

Streptavidin MagneSphere® Paramagnetic Particles

 Z548F 3 × 3ml

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

CC

製品仕様

You are viewing: Z5300 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Nuclease-Free Water

 P119C 2 × 25ml View Product

SSC, 20X

 Z525A 2 × 1.4ml

Biotinylated Oligo(dT) Probe

 Z526B 1 × 50μl View Product

MagneSphere® Technology Magnetic Separation Stand (two-position)

 Z5332 1 × 1.5ml

Streptavidin MagneSphere® Paramagnetic Particles

 Z548C 15 × 0.6ml

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

CC

製品仕様

You are viewing: Z5310 Change Configuration

選択製品の構成品内容

Item Part # Size Available Separately

Nuclease-Free Water

 P119C 2 × 25ml View Product

SSC, 20X

 Z525A 2 × 1.4ml

Biotinylated Oligo(dT) Probe

 Z526B 1 × 50μl View Product

Streptavidin MagneSphere® Paramagnetic Particles

 Z548C 15 × 0.6ml

SDS

Search for SDS

分析証明書

Search by lot number

使用制限

For Research Use Only. Not for Use in Diagnostic Procedures.

保存条件

CC

技術資料

サイテーション

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SV Total RNA Isolation System

組織、細胞および白血球より簡便な遠心法または吸引法で total RNA を精製

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関連製品

Streptavidin MagneSphere® Paramagnetic Particles

ビオチン化された核酸またはタンパク質の磁力による分離および精製

Z5481, Z5482

MagneSphere® Technology Magnetic Separation Stands

磁力による生体分子の分離に適した強力な希土類磁石

Z5331, Z5332, Z5333, Z5341, Z5342, Z5343

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