PolyATtract® System 1000
Isolates mRNA Directly from Crude Cell or Tissue Lysates
- mRNA Purification is complete 45 minutes or less
- Works with tissue amounts from 5mg–2g per isolation
- No lengthy ethanol precipitation steps, phenol:chloroform extractions, and overnight ultracentrifugation
- Magnetic separation stand (Cat.# Z5410) accommodates 1.5ml, 2ml, 15ml and 50ml tube sizes
Catalog Number:
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Size
Catalog Number: Z5420
Catalog Number: Z5400
The PolyATtract® System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolations. This system uses the MagneSphere® technology for the purification of poly(A)+ RNA, eliminating the need for oligo(dT) cellulose columns. The increased yield of mRNA using this system allows the detection of low-copy-number mRNAs in relatively small amounts of material using Northern blot analysis. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation, cDNA synthesis, PCR analysis, ribonuclease (RNase) protection assays, primer extension and Northern blots.
The MagneSphere® Technology Magnetic Separation Stands can be used in conjunction with any of the PolyATtract® Systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules.
Protocols
Complete Protocol
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Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
|
GTC Extraction Buffer |
Z522A | 1 × 20ml | |
|
Biotinylated Oligo(dT) Probe |
Z526B | 1 × 50μl | View Product |
|
Dilution Buffer |
Z536A | 1 × 40ml | |
|
mRNA User Tubes |
Z537A | 4 × 10 each | |
|
0.5X SSC Solution |
Z538B | 3 × 125ml | |
|
PolyATtract® System 1000 Magnetic Separation Stand |
Z541A | 1 × 1 each | View Product |
|
Streptavidin MagneSphere® Paramagnetic Particles |
Z548D | 1 × 25ml | |
|
Beta-Mercaptoethanol |
Z559A | 1 × 900μl |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
|
GTC Extraction Buffer |
Z522A | 1 × 20ml | |
|
Biotinylated Oligo(dT) Probe |
Z526B | 1 × 50μl | View Product |
|
Dilution Buffer |
Z536A | 1 × 40ml | |
|
mRNA User Tubes |
Z537A | 4 × 10 each | |
|
0.5X SSC Solution |
Z538B | 3 × 125ml | |
|
Streptavidin MagneSphere® Paramagnetic Particles |
Z548D | 1 × 25ml | |
|
Beta-Mercaptoethanol |
Z559A | 1 × 900μl |
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