ReliaPrep™ miRNA Cell and Tissue Miniprep System
Fast, Phenol-Free miRNA Isolation from Cells and Tissues
- Isolate concentrated, quality RNA in only 40 minutes
- Eliminate harsh organic reagents
- Work with small samples and low elution volumes
Catalog Number:
Size
Catalog Number: Z6210
Catalog Number: Z6211
Catalog Number: Z6212
高濃度のRNAを迅速、安全に精製
これまで有機溶媒を使用した精製法が最も感度の高い RNA 精製法として受け入れられていますが、ReliaPrep™ miRNA purification system は実質的に同等の収量と純度で増幅可能な miRNAを精製でき、フェノール : クロロホルム抽出ステップが無く、有害な有機溶媒の取扱いや廃棄も不要です。
miRNA を含むRNA を高濃度で精製
RNA Species from Cells
Expression of miRNA (miR16), snoRNA (U6), and sncRNA (RNU44 and RNU48) isolated from HEK293 cells.
RNA Species from Tissue
Expression of miRNA and snoRNA products detected from total RNA isolated from 10mg of frozen mouse liver and kidney tissue.
少量のサンプルからでもRNAを精製
RNA from 10–105cells
Here, the miRNA targets U6 and miR16 were amplified after RNA isolation from varying cell culture inputs. RNA was purified from as few as 10 cells to as many as 1x105 HEK293 cells. miR16 and U6 expression levels were determined using qPCR.
RNA from 0.0032–10mg Tissue
In this example, the miRNA targets let7e and miR16 were reliably amplified from ReliaPrep-purified RNA isolated from 0.0032–10mg frozen mouse kidney tissue. miR16 and let7e expression levels were determined by qPCR.
フェノール : クロロホルム抽出の無い高純度 RNA 精製
RNA purity A260/A280
All kits purified RNA with A260/A280 purity ratios in the accepted 1.8–2.2 range.
RNA purity A260/A230 ratios
Kits using phenol chloroform reagents generated RNA with A260/A230 ratios below the accepted 1.8–2.2 range.
技術ポスターの紹介
MicroRNA Analysis Paired with a Novel Live Cell Viability Assay: A Complete Epigenetic Workflow in Human Cancer Cell Lines
このポスターでは増殖および転移するがん細胞の低分子あるいはノンコーディング RNA の役割を調べるために Reliaprep™ miRNA system で単離した total RNA、mRNA および miRNA を使用しています。
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 2 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 2 × 10/pack | |
| 1-Thioglycerol | A208A | 2 × 75μl | |
DNase Buffer, 10X |
M198B | 1 × 500μl | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
Lysis Buffer (LBA) |
Z106A | 1 × 6ml | |
|
Elution Tubes |
Z200B | 1 × 25/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372C | 1 × 9ml |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | |
ReliaPrep™ Minicolumns |
A207B | 2 × 50/pack | |
|
1-Thioglycerol |
A208B | 1 × 900μl | |
DNase Buffer, 10X |
M198B | 1 × 500μl | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) |
Z106B | 1 × 30ml | |
|
Elution Tubes |
Z200C | 2 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z3091 | 1 × 58.8ml | View Product |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372A | 1 × 44ml |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 10 × 50/pack | |
|
1-Thioglycerol |
A208B | 4 × 900μl | |
DNase Buffer, 10X |
M198B | 3 × 500μl | |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
Lysis Buffer (LBA) |
Z106C | 1 × 150ml | |
|
Elution Tubes |
Z200C | 10 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
DNase I (lyophilized) |
Z358A | 5 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372B | 1 × 220ml |
Resources
技術記事
- RNA Isolation from 3D Microtissue Cultures: A Comparison of Manual and Automated Methods
- microRNA Purification from Plant Tissues Using the ReliaPrep™ miRNA Cell and Tissue Miniprep System
- Total RNA Isolation from 3D Cell Cultures or Cells in Matrigel® Matrix Using the ReliaPrep™ miRNA Cell and Tissue Miniprep System
Related Products
類似製品
Maxwell® RSC miRNA from Tissue or Plasma and Serum Kits
In combination with the Maxwell® RSC Instrument, the kit purifies high-quality total RNA with an enhanced miRNA enrichment.
AS1460, AS1680
ReliaPrep™ FFPE Total RNA Miniprep System
有機溶媒や一昼夜の消化が不要な FFPE 組織からの増幅可能な total RNA 精製
Z1001, Z1002
ReliaPrep™ RNA Miniprep Systems
わずか100個の細胞または0.25mgの組織からTotal RNAを抽出。30分の簡単なプロトコール。
Z6014, Z6015, Z6010, Z6011, Z6012, Z6110, Z6111, Z6112
