ReliaPrep™ RNA Miniprep Systems
Efficiently Purify RNA from as Few as 100 Cells or 0.25mg Tissue
- Simple, 30-minute protocol
- Elutes in small volume, generating highly concentrated RNA
- No phenol/chloroform or β-mercaptoethanol required
Catalog Number:
Choose a sample type
Size
Catalog Number: Z6010
Catalog Number: Z6011
Catalog Number: Z6012
Catalog Number: Z6110
Catalog Number: Z6111
Catalog Number: Z6112
貴重な細胞や組織サンプルより高純度なRNAを高濃度に精製
- 100個程度の少ない細胞あるいは0.25～20mg程度の少量の組織からでもRNAを精製可能
- 阻害剤を含まない純度の高いRNAを精製
- 15µl以下に濃縮されたRNAを溶出
- 迅速な30分間プロトコル
- 様々な下流の用途（定量など）に適したRNAの精製
【簡易マニュアル】日本語：cell,【簡易マニュアル】日本語：tissue
RNA from Cells
高収量
ReliaPrep™ Cell RNA Miniprep Systemは 100 から 1 x 106 個の HeLa 細胞より他社競合製品よりも多くのRNAを効率的に精製（右グラフ）。4種の細胞株それぞれ100,000細胞からの RNA 収量（下グラフ）。ReliaPrep™ System のその他のパフォーマンスデータについては Application Note をご覧ください。
RNA from Tissues
組織から精製した RNA の収量および純度
ReliaPrep™ RNA Tissue Miniprep System を用いて線維性および非線維性のマウス組織より精製した標準的なRNA 収量。RNA は様々なマウス組織 10mg より精製。収量および純度は吸光光度計 Nanodrop® 1000 を用いて測定した。
ReliaPrep™ RNA Tissue Miniprep System により得られたその他のデータについては Application Note をご覧ください。
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 2 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
| 1-Thioglycerol | A208A | 1 × 75μl | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
|
BL Buffer |
Z103A | 2 × 3.25ml | |
|
Elution Tubes |
Z200B | 1 × 25/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
| Yellow Core Buffer | Z317B | 1 × 300μl | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | |
|
1-Thioglycerol |
A208B | 1 × 900μl | |
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
|
BL Buffer |
Z103B | 1 × 32.5ml | |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
| RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
|
Yellow Core Buffer |
Z317A | 1 × 2.5ml | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
1-Thioglycerol |
A208B | 2 × 900μl | |
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
| BL Buffer | Z103C | 1 × 150ml | |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
Yellow Core Buffer |
Z317C | 1 × 11ml | |
|
MnCl2, 0.09M |
Z318B | 2 × 750μl | |
|
DNase I (lyophilized) |
Z358A | 3 × 1 vial | View Product |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130C | 2 × 10/pk | |
| ReliaPrep™ Minicolumns | A207A | 1 × 10/pack | |
| 1-Thioglycerol | A208A | 2 × 75μl | |
Column Wash Solution (CWE) |
A254A | 1 × 1ml | |
|
Nuclease-Free Water |
P119A | 1 × 1,250μl | View Product |
Lysis Buffer (LBA) |
Z106A | 1 × 6ml | |
|
Elution Tubes |
Z200B | 1 × 25/pk | |
|
RNA Wash Solution (RWA) |
Z309B | 1 × 11.8ml | View Product |
| Yellow Core Buffer | Z317B | 1 × 300μl | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358B | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372C | 1 × 9ml |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | |
|
1-Thioglycerol |
A208B | 2 × 900μl | |
Column Wash Solution (CWE) |
A254B | 1 × 5ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) |
Z106B | 1 × 30ml | |
|
Elution Tubes |
Z200C | 1 × 50/pk | |
| RNA Wash Solution (RWA) | Z309E | 1 × 35ml | View Product |
|
Yellow Core Buffer |
Z317A | 1 × 2.5ml | |
|
MnCl2, 0.09M |
Z318A | 1 × 250μl | |
|
DNase I (lyophilized) |
Z358A | 1 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372A | 1 × 44ml |
選択製品の構成品内容
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Collection Tubes |
A130B | 2 × 250/pk | |
ReliaPrep™ Minicolumns |
A207B | 5 × 50/pack | |
|
1-Thioglycerol |
A208B | 4 × 900μl | |
Column Wash Solution (CWE) |
A254C | 1 × 24ml | |
|
Nuclease-Free Water |
P119E | 1 × 13ml | View Product |
Lysis Buffer (LBA) |
Z106C | 1 × 150ml | |
|
Elution Tubes |
Z200C | 5 × 50/pk | |
|
RNA Wash Solution (RWA) |
Z309D | 1 × 206ml | View Product |
|
Yellow Core Buffer |
Z317C | 1 × 11ml | |
|
MnCl2, 0.09M |
Z318B | 2 × 750μl | |
|
DNase I (lyophilized) |
Z358A | 3 × 1 vial | View Product |
|
RNA Dilution Buffer (RDB) |
Z372B | 1 × 220ml |
Resources
Related Products
類似製品
関連製品
RNasin® Ribonuclease Inhibitor
真核生物由来RNaseの広範な阻害において最も信頼されている製品
N2111, N2115, N2511, N2515, N2611, N2615
Nuclease-Free Water
DNase、RNase 活性フリーの水
MC1191, P1193, P1195, P1197, P1199
RNA Markers
281–6,583塩基を生じる、特殊なテンプレートからin vitroで合成された9本のRNA転写産物のラダー
G3191
DNase I (Lyophilized)
RNA精製キットのコンポーネントで、精製工程でのDNAコンタミネーションの除去に使用
Z3585
